Fat accumulation in the liver of obese rats is alleviated by soy protein isolate through β-catenin signaling.
Fat accumulation in the liver of obese rats is alleviated by soy protein isolate through β-catenin signaling.
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作者：
Zhou, Dan;Lezmi, Stephane;Wang, Huan;Davis, Jeremy;Banz, William;Chen, Hong
本研究旨在探讨SPI对肥胖大鼠肝脏Wnt/β-catenin信号通路的影响，以及该通路在调节肝脏脂肪积累中的作用。肥胖和瘦的 Zucker 大鼠被喂食含有酪蛋白或 SPI 作为蛋白质来源的饮食 17 周。对肝脏样本进行组织学和生化分析、实时PCR、蛋白质印迹、免疫染色、短干扰RNA测定。我们的研究表明，与酪蛋白喂养的对照相比，SPI 喂养的肥胖大鼠肝脏中的脂肪含量显着降低，同时肝细胞空泡减少。与瘦组相比，肥胖大鼠肝脏中的β-连环蛋白水平下调，表明肥胖基因型的Wnt信号传导总体降低。重要的是，喂食 SPI 饮食可以减轻肥胖大鼠体内 β-连环蛋白的抑制。对大鼠肝癌细胞进行 siRNA 处理证实，β-连环蛋白的沉默会加剧脂肪酸诱导的脂肪积累，这表明 Wnt/β-连环蛋白信号在肝脂肪代谢中发挥着重要作用。 SPI 摄入恢复了 β-连环蛋白信号传导，减轻了肥胖大鼠的肝脏脂肪堆积和肝损伤。
The current study aim to investigate the effects of SPI on Wnt/β-catenin signaling in the liver of obese rats, as well as the roles of this pathway in regulating the hepatic fat accumulation. Obese and lean Zucker rats were fed diets containing either casein or SPI as protein source for 17 weeks. Histology and biochemical analysis, real-time PCR, Western blot, immunostaining, short interfering RNA assay were performed for liver samples. Our study showed that fat content was significantly lowered in the liver of SPI-fed obese rats, accompanied by a reduction in hepatocellular vacuolation, compared to the casein-fed control. β-catenin protein level in the liver of obese rats was down-regulated compared to the lean group, indicating that the obese genotype exhibits an overall reduction in Wnt signaling. Importantly the repression of β-catenin in the obese rats was alleviated by feeding the SPI diet. siRNA treatment in rat hepatoma cells confirmed that silencing of β-catenin exacerbated fatty acid-induced fat accumulation, which implicated an important function of Wnt/β-catenin signaling in hepaticfat metabolism. SPI intake restored β-catenin signaling and alleviated hepatic fat accumulation and liver damage in the obese rats.
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影响因子：
3.7
作者：
Chen JR;Lazarenko OP;Wu X;Tong Y;Blackburn ML;Shankar K;Badger TM;Ronis MJ
通讯作者：
Ronis MJ
影响因子：
5.8
作者：
Kumar, S;Leontovich, A;Bahn, RS
通讯作者：
Bahn, RS
影响因子：
4.2
作者：
NAGATA, Y;ISHIWAKI, N;SUGANO, M
通讯作者：
SUGANO, M
DOI：
10.3350/kjhep.2009.15.s6.s34
发表时间：
2009-12-01
期刊：
The Korean journal of hepatology
影响因子：
--
作者：
Park, Sang Hoon
通讯作者：
Park, Sang Hoon
DOI：
10.1016/j.jnutbio.2011.07.002
发表时间：
2012-10
期刊：
The Journal of nutritional biochemistry
影响因子：
--
作者：
Liu Z;Brooks RS;Ciappio ED;Kim SJ;Crott JW;Bennett G;Greenberg AS;Mason JB
通讯作者：
Mason JB