Effector caspase Dcp-1 and IAP protein Bruce regulate starvation-induced autophagy during Drosophila melanogaster oogenesis.
Effector caspase Dcp-1 and IAP protein Bruce regulate starvation-induced autophagy during Drosophila melanogaster oogenesis.
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DOI：
10.1083/jcb.200712091
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发表时间：
2008-09-22
影响因子：
7.8
通讯作者：
Gorski, Sharon M.
中科院分区：
文献类型：
--
作者：
Hou, Ying-Chen Claire;Chittaranjan, Suganthi;Barbosa, Sharon Gonzalez;McCall, Kimberly;Gorski, Sharon M.
自噬和凋亡之间存在复杂的关系，但其相互作用的调控机制在很大程度上是未知的。我们对果蝇细胞死亡相关基因进行了系统的研究，以确定它们在饥饿诱导的自噬调控中的需求。我们发现六个细胞死亡基因--死亡半胱天冬酶-1（Dcp-1）、hid、布鲁斯、Buffy、debcl和p53--以及Ras-Raf-丝裂原活化蛋白激酶信号通路组分在D.黑腹培养细胞。在D.在黑腹鱼卵子发生中，我们发现自噬在两个营养状态检查点被诱导：生殖细胞和中期卵子发生。在这两个阶段，效应器半胱天冬酶Dcp-1和凋亡蛋白布鲁斯的抑制剂的功能，以调节自噬和饥饿诱导的细胞死亡。Atg 1和Atg 7的突变导致退化中期卵室的DNA片段减少，但似乎不影响核凝聚，这表明自噬部分导致卵巢细胞死亡。我们的研究提供了新的见解的分子机制，协调调节自噬和凋亡事件在体内。
A complex relationship exists between autophagy and apoptosis, but the regulatory mechanisms underlying their interactions are largely unknown. We conducted a systematic study of Drosophila melanogaster cell death–related genes to determine their requirement in the regulation of starvation-induced autophagy. We discovered that six cell death genes—death caspase-1 (Dcp-1), hid, Bruce, Buffy, debcl, and p53—as well as Ras–Raf–mitogen activated protein kinase signaling pathway components had a role in autophagy regulation in D. melanogaster cultured cells. During D. melanogaster oogenesis, we found that autophagy is induced at two nutrient status checkpoints: germarium and mid-oogenesis. At these two stages, the effector caspase Dcp-1 and the inhibitor of apoptosis protein Bruce function to regulate both autophagy and starvation-induced cell death. Mutations in Atg1 and Atg7 resulted in reduced DNA fragmentation in degenerating midstage egg chambers but did not appear to affect nuclear condensation, which indicates that autophagy contributes in part to cell death in the ovary. Our study provides new insights into the molecular mechanisms that coordinately regulate autophagic and apoptotic events in vivo.
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DOI：
10.1083/jcb.152.4.657
发表时间：
2001-02-19
期刊：
The Journal of cell biology
影响因子：
--
作者：
Mizushima N;Yamamoto A;Hatano M;Kobayashi Y;Kabeya Y;Suzuki K;Tokuhisa T;Ohsumi Y;Yoshimori T
通讯作者：
Yoshimori T
影响因子：
11.8
作者：
Arama, E;Agapite, J;Steller, H
通讯作者：
Steller, H
DOI：
10.1073/pnas.0400443101
发表时间：
2004-03-09
影响因子：
11.1
作者：
Mills, KR;Reginato, M;Brugge, JS
通讯作者：
Brugge, JS
影响因子：
10.5
作者：
Juhasz, Gabor;Erdi, Balazs;Neufeld, Thomas P.
通讯作者：
Neufeld, Thomas P.
影响因子：
3.9
作者：
Ferraro, Efisabetta;Cecconi, Francesco
通讯作者：
Cecconi, Francesco