Nanoshell-mediated photothermal therapy improves survival in a murine glioma model.
Nanoshell-mediated photothermal therapy improves survival in a murine glioma model.
复制标题
DOI：
10.1007/s11060-010-0470-8
复制
发表时间：
2011-08
影响因子：
3.9
通讯作者：
West, Jennifer L.
中科院分区：
文献类型：
--
作者：
Day, Emily S.;Thompson, Patrick A.;Zhang, Linna;Lewinski, Nastassja A.;Ahmed, Nabil;Drezek, Rebekah A.;Blaney, Susan M.;West, Jennifer L.
我们正在开发一种针对高级神经胶质瘤的新型治疗方法，使用近红外吸收的二氧化硅-金纳米壳，这些纳米壳在暴露于近红外激光时会被热激活，从而不可逆地损害癌细胞。这项工作的目标是确定纳米壳介导的光热疗法在小鼠异种移植模型中的体内疗效。通过皮下植入萤火虫荧光素酶标记的 U373 人神经胶质瘤细胞，在雄性 Icr-Tac:ICR-PrkdcSCID 小鼠中诱导肿瘤，并进行生物分布和存活研究。为了评估纳米颗粒的生物分布，将纳米壳静脉注射到荷瘤小鼠体内，6、24或48小时后，通过电感耦合等离子体质谱（ICP-MS）和组织学评估肿瘤、肝脏、脾脏、大脑、肌肉和血液的金含量。肿瘤中的纳米壳浓度在前 24 小时内增加，此后稳定。通过静脉内输送盐水或纳米壳并在注射后 24 小时用近红外激光外部照射肿瘤来评估治疗效果。通过监测激光照射后小鼠的肿瘤大小、肿瘤发光和存活时间来评估治疗的成功。与对照组相比，纳米壳治疗组的存活率显着提高（P < 0.02），并且纳米壳治疗组中 57% 的小鼠在 90 天的研究期结束时仍然没有肿瘤。相比之下，对照组中没有一只小鼠存活超过 24 天，平均存活期仅为 13.3 天。这些研究的结果表明，纳米壳介导的光热疗法代表了一种有前途的恶性神经胶质瘤的新型治疗策略。
We are developing a novel treatment for high-grade gliomas using near infrared-absorbing silica–gold nanoshells that are thermally activated upon exposure to a near infrared laser, thereby irreversibly damaging cancerous cells. The goal of this work was to determine the efficacy of nanoshell-mediated photothermal therapy in vivo in murine xenograft models. Tumors were induced in male Icr-Tac:ICR-PrkdcSCID mice by subcutaneous implantation of Firefly Luciferase-labeled U373 human glioma cells and biodistribution and survival studies were performed. To evaluate nanoparticle biodistribution, nanoshells were delivered intravenously to tumor-bearing mice and after 6, 24, or 48 h the tumor, liver, spleen, brain, muscle, and blood were assessed for gold content by inductively coupled plasma-mass spectrometry (ICP-MS) and histology. Nanoshell concentrations in the tumor increased for the first 24 h and stabilized thereafter. Treatment efficacy was evaluated by delivering saline or nanoshells intravenously and externally irradiating tumors with a near infrared laser 24 h post-injection. Success of treatment was assessed by monitoring tumor size, tumor luminescence, and survival time of the mice following laser irradiation. There was a significant improvement in survival for the nanoshell treatment group versus the control (P < 0.02) and 57% of the mice in the nanoshell treatment group remained tumor free at the end of the 90-day study period. By comparison, none of the mice in the control group survived beyond 24 days and mean survival was only 13.3 days. The results of these studies suggest that nanoshell-mediated photothermal therapy represents a promising novel treatment strategy for malignant glioma.
登录
查看更多内容
影响因子：
158.5
作者：
Stupp, R;Mason, WP;Ryan, G
通讯作者：
Ryan, G
DOI：
10.1073/pnas.2232479100
发表时间：
2003-11-11
影响因子：
11.1
作者：
Hirsch, LR;Stafford, RJ;West, JL
通讯作者：
West, JL
影响因子：
3.9
作者：
Jordan, A;Scholz, R;Felix, R
通讯作者：
Felix, R
影响因子：
3.6
作者：
Loo, C;Hirsch, L;Drezek, R
通讯作者：
Drezek, R
影响因子：
11.2
作者：
Schwartz, Jon A.;Shetty, Anil M.;Payne, J. Donald
通讯作者：
Payne, J. Donald