Recruitment of the autophagic machinery to endosomes during infection is mediated by ubiquitin.
Recruitment of the autophagic machinery to endosomes during infection is mediated by ubiquitin.
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DOI：
10.1083/jcb.201304188
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发表时间：
2013-10-14
期刊：
影响因子：
--
通讯作者：
Yoshimori T
中科院分区：
文献类型：
--
作者：
Fujita N;Morita E;Itoh T;Tanaka A;Nakaoka M;Osada Y;Umemoto T;Saitoh T;Nakatogawa H;Kobayashi S;Haraguchi T;Guan JL;Iwai K;Tokunaga F;Saito K;Ishibashi K;Akira S;Fukuda M;Noda T;Yoshimori T
在细菌入侵后，泛素与宿主内体蛋白结合，并被独立于LC 3的自噬机制识别。虽然泛素被认为是重要的自噬隔离入侵细菌（也称为xenophagy），其确切的作用仍然在很大程度上是谜。在这里，我们确定了泛素是如何参与这一过程的。入侵后，泛素与内含体中的宿主细胞蛋白结合，内含体含有沙门氏菌或转染试剂包被的乳胶（聚苯乙烯）珠，其模拟入侵细菌。泛素被自噬机制识别，独立于LC 3-泛素通过衔接蛋白的相互作用，包括泛素和Atg 16 L1之间的直接相互作用。为了确保入侵的病原体被捕获和降解，Atg 16 L1靶向通过两个备用系统来确保，这两个备用系统将Atg 16 L1锚到泛素修饰的内体。因此，我们揭示，泛素是一个关键的分子，连接含细菌的内体与自噬机制上游的LC 3。
After bacterial invasion, ubiquitin is conjugated to host endosomal proteins and recognized by the autophagic machinery independent of LC3. Although ubiquitin is thought to be important for the autophagic sequestration of invading bacteria (also called xenophagy), its precise role remains largely enigmatic. Here we determined how ubiquitin is involved in this process. After invasion, ubiquitin is conjugated to host cellular proteins in endosomes that contain Salmonella or transfection reagent–coated latex (polystyrene) beads, which mimic invading bacteria. Ubiquitin is recognized by the autophagic machinery independently of the LC3–ubiquitin interaction through adaptor proteins, including a direct interaction between ubiquitin and Atg16L1. To ensure that invading pathogens are captured and degraded, Atg16L1 targeting is secured by two backup systems that anchor Atg16L1 to ubiquitin-decorated endosomes. Thus, we reveal that ubiquitin is a pivotal molecule that connects bacteria-containing endosomes with the autophagic machinery upstream of LC3.
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LRR和环域蛋白LRSAM1是一种E3连接酶，对于细胞内沙门氏菌的泛素依赖性自噬至关重要。
影响因子：
30.3
作者：
Huett A;Heath RJ;Begun J;Sassi SO;Baxt LA;Vyas JM;Goldberg MB;Xavier RJ
通讯作者：
Xavier RJ
影响因子：
64.5
作者：
Katzmann, DJ;Babst, M;Emr, SD
通讯作者：
Emr, SD
DOI：
10.1083/jcb.124.5.677
发表时间：
1994-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Desjardins M;Huber LA;Parton RG;Griffiths G
通讯作者：
Griffiths G
影响因子：
4
作者：
Mizushima, N;Kuma, A;Yoshimori, T
通讯作者：
Yoshimori, T
影响因子：
13.3
作者：
Hara, Taichi;Mizushima, Noboru
通讯作者：
Mizushima, Noboru