Yeast targets for mRNA methylation.
Yeast targets for mRNA methylation.
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DOI：
10.1093/nar/gkq266
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发表时间：
2010-09
影响因子：
14.9
通讯作者：
Fray RG
中科院分区：
文献类型：
--
作者：
Bodi Z;Button JD;Grierson D;Fray RG
N6 - 甲基腺苷（m6A）是一种修饰碱基，存在于所有高等真核生物以及酿酒酵母（Saccharomyces cerevisiae）的mRNA中，在酿酒酵母的孢子形成过程中，m6A水平会升高。甲基转移酶Ime4负责这种修饰，并在减数分裂起始过程中发挥作用。然而，这种核苷酸修饰的功能以及分布范围都尚未确定。我们证明，在酿酒酵母中，在孢子形成细胞的mRNA中，在GpA环境下存在大量的内部腺苷甲基化，这与高等真核生物偏好的甲基化共有序列一致。根据我们的定量数据，在减数分裂过程中，每两条转录本可能就有一条含有一个m6A。由于甲基化分布在所有mRNA大小范围内，所以m6A可能不限于一小部分mRNA。我们开发了一种基于抗体的新方法来鉴定含有m6A的mRNA，利用这种方法，鉴定出减数分裂的三个关键早期调控因子IME1、IME2和IME4自身的转录本是被甲基化的。IME2中m6A的位置被缩小到3′端的一个区域。这些以及其他靶点的甲基化表明了IME4可能控制导致减数分裂的发育选择的机制。
N6-Methyladenosine (m6A) is a modified base present in the mRNA of all higher eukaryotes and in Saccharomyces cerevisiae, where there is an increase in m6A levels during sporulation. The methyltransferase, Ime4, is responsible for this modification and has a role in the initiation of meiosis. However, neither the function, nor the extent of distribution of this nucleotide modification is established. We demonstrate that in S. cerevisiae, substantial levels of internal adenosine methylation are present in the GpA context in mRNA from sporulating cells, which is consistent with the preferred methylation consensus of higher eukaryotes. Based upon our quantification data, every second transcript could contain one m6A during meiosis. As methylation is distributed across all mRNA size ranges, it is likely that m6A is not limited to a small population of messages. We developed a new antibody based method for identifying m6A containing messages, and using this method the transcripts of three key, early regulators of meiosis, IME1, IME2 and IME4 itself, were identified as being methylated. The position of m6A in IME2 was narrowed down to a region in the 3′-end. Methylation of these and other targets suggests mechanisms by which IME4 could control developmental choices leading to meiosis.
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影响因子：
14.9
作者：
Clancy, MJ;Shambaugh, ME;Bokar, JA
通讯作者：
Bokar, JA
影响因子：
7.7
作者：
Houseley, J;Tollervey, D
通讯作者：
Tollervey, D
影响因子：
5.4
作者：
ALONI, Y;DHAR, R;KHOURY, G
通讯作者：
KHOURY, G
DOI：
10.1139/o79-112
发表时间：
1979-01-01
期刊：
CANADIAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
KENNEDY, TD;LANE, BG
通讯作者：
LANE, BG
DOI：
10.1111/j.1432-1033.1980.tb04425.x
发表时间：
1980-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
HAUGLAND, RA;CLINE, MG
通讯作者：
CLINE, MG