Gene Expression Analysis of Alfalfa Seedlings Response to Acid-Aluminum.
Gene Expression Analysis of Alfalfa Seedlings Response to Acid-Aluminum.
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苜蓿幼苗对酸铝反应的基因表达分析
DOI：
10.1155/2016/2095195
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发表时间：
2016
影响因子：
2.9
通讯作者：
An Y
中科院分区：
文献类型：
--
作者：
Zhou P;Su L;Lv A;Wang S;Huang B;An Y
酸铝 (Al) 对植物有毒，极大地影响全世界的作物生产。为了了解植物对酸性土壤和铝毒性的反应，我们使用经过酸铝处理的苜蓿幼苗的微阵列数据检查了整体基因表达。在 pH 4.5 处理下，有 3,926 个基因在 Al3+ 离子的作用下显着上调或下调，其中 66.33% 在根中发现。其功能类别主要涉及植物激素调节、活性氧和转运蛋白。基因本体（GO）富集和KEGG分析均表明苯丙素生物合成、苯丙氨酸代谢和类黄酮生物合成在苜蓿防御铝胁迫中发挥着关键作用。此外，我们发现MYB和WRKY家族蛋白等转录因子也可能参与活性氧反应和类黄酮生物合成的调节。因此，全局基因表达谱的发现为了解苜蓿中植物防御酸铝胁迫的机制提供了见解。了解关键的调控基因和途径将有利于提高作物产量，不仅是苜蓿，还有酸铝胁迫下的其他作物。
Acid-Aluminum (Al) is toxic to plants and greatly affects crop production worldwide. To understand the responses of plants to acid soils and Aluminum toxicity, we examined global gene expression using microarray data in alfalfa seedlings with the treatment of acid-Aluminum. 3,926 genes that were identified significantly up- or downregulated in response to Al3+ ions with pH 4.5 treatment, 66.33% of which were found in roots. Their functional categories were mainly involved with phytohormone regulation, reactive oxygen species, and transporters. Both gene ontology (GO) enrichment and KEGG analysis indicated that phenylpropanoid biosynthesis, phenylalanine metabolism, and flavonoid biosynthesis played a critical role on defense to Aluminum stress in alfalfa. In addition, we found that transcription factors such as the MYB and WRKY family proteins may be also involved in the regulation of reactive oxygen species reactions and flavonoid biosynthesis. Thus, the finding of global gene expression profile provided insights into the mechanisms of plant defense to acid-Al stress in alfalfa. Understanding the key regulatory genes and pathways would be advantageous for improving crop production not only in alfalfa but also in other crops under acid-Aluminum stress.
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影响因子：
2.5
作者：
An, Yuan;Zhou, Peng;Shi, Dongyan
通讯作者：
Shi, Dongyan
agriGO：农业界的 GO 分析工具包
影响因子：
14.9
作者：
Du Z;Zhou X;Ling Y;Zhang Z;Su Z
通讯作者：
Su Z
影响因子：
5.3
作者：
Mattiello L;Kirst M;da Silva FR;Jorge RA;Menossi M
通讯作者：
Menossi M
影响因子：
6.5
作者：
Heidarabadi, Maryam Dahajipour;Ghanati, Faezeh;Fujiwara, Toru
通讯作者：
Fujiwara, Toru
DOI：
10.1073/pnas.250379897
发表时间：
2000-12-05
影响因子：
11.1
作者：
Grotewold, E;Sainz, MB;Chandler, VL
通讯作者：
Chandler, VL