Transcriptional profile of maize roots under acid soil growth.
Transcriptional profile of maize roots under acid soil growth.
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DOI：
10.1186/1471-2229-10-196
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发表时间：
2010-09-09
影响因子：
5.3
通讯作者：
Menossi M
中科院分区：
文献类型：
--
作者：
Mattiello L;Kirst M;da Silva FR;Jorge RA;Menossi M
铝毒是世界范围内许多作物最重要的产量限制因子之一。铝毒综合征的主要症状是根系生长受到抑制，导致水分和养分吸收不良。铝耐受性已经使用水培实验进行了广泛的研究。然而，与土壤条件不同的是，这种方法并不能解决根系正常生长和发育所需的所有成分。在本研究中，我们种植了两个玉米基因型与对比耐铝在土壤中含有有毒水平的铝，然后比较他们的转录组反应。在酸性土壤中，铝敏感基因型（S1587-17）比耐铝基因型（Cat 100 -6）表现出更强的根系生长抑制、更多的铝积累和更多的胼胝质沉积。转录组分析显示，在酸性土壤中生长的S1587-17中差异表达的基因数量较多，这可能是由于铝毒性的次级效应。参与有机酸生物合成的基因，这是经常与铝耐受性反应，没有差异调节后，在两种基因型酸性土壤暴露。而与生长素、乙烯和木质素生物合成相关的基因在铝敏感基因型中表达上调，表明这些途径可能与根系生长抑制有关。通过比较这两个玉米品系，我们能够发现仅在耐铝品系中上调的基因，其也呈现出比在铝敏感品系中观察到的那些更高的绝对水平。这些基因编码脂肪酶水解酶、视黄醇脱氢酶、富含甘氨酸的蛋白质、WRKY转录家族成员和两个未知蛋白质。这项工作提供了第一个表征的生理和转录反应的玉米根生长在酸性土壤中含有有毒水平的铝。转录组配置文件突出了几个途径，在酸性土壤中生长过程中铝的毒性和耐受性。我们发现了几个在以前的研究中没有发现的基因，使用水培实验，增加了我们对植物对酸性土壤反应的理解。使用两个具有显着不同的铝耐受性的种质允许鉴定的基因，是一个有价值的工具，用于评估在酸性土壤中的玉米耐铝的机制。
Aluminum (Al) toxicity is one of the most important yield-limiting factors of many crops worldwide. The primary symptom of Al toxicity syndrome is the inhibition of root growth leading to poor water and nutrient absorption. Al tolerance has been extensively studied using hydroponic experiments. However, unlike soil conditions, this method does not address all of the components that are necessary for proper root growth and development. In the present study, we grew two maize genotypes with contrasting tolerance to Al in soil containing toxic levels of Al and then compared their transcriptomic responses. When grown in acid soil containing toxic levels of Al, the Al-sensitive genotype (S1587-17) showed greater root growth inhibition, more Al accumulation and more callose deposition in root tips than did the tolerant genotype (Cat100-6). Transcriptome profiling showed a higher number of genes differentially expressed in S1587-17 grown in acid soil, probably due to secondary effects of Al toxicity. Genes involved in the biosynthesis of organic acids, which are frequently associated with an Al tolerance response, were not differentially regulated in both genotypes after acid soil exposure. However, genes related to the biosynthesis of auxin, ethylene and lignin were up-regulated in the Al-sensitive genotype, indicating that these pathways might be associated with root growth inhibition. By comparing the two maize lines, we were able to discover genes up-regulated only in the Al-tolerant line that also presented higher absolute levels than those observed in the Al-sensitive line. These genes encoded a lipase hydrolase, a retinol dehydrogenase, a glycine-rich protein, a member of the WRKY transcriptional family and two unknown proteins. This work provides the first characterization of the physiological and transcriptional responses of maize roots when grown in acid soil containing toxic levels of Al. The transcriptome profiles highlighted several pathways that are related to Al toxicity and tolerance during growth in acid soil. We found several genes that were not found in previous studies using hydroponic experiments, increasing our understanding of plant responses to acid soil. The use of two germplasms with markedly different Al tolerances allowed the identification of genes that are a valuable tool for assessing the mechanisms of Al tolerance in maize in acid soil.
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影响因子：
3.8
作者：
Chaffai, R;Marzouk, B;El Ferjani, E
通讯作者：
El Ferjani, E
影响因子：
7.4
作者：
DELHAIZE, E;RYAN, PR;RANDALL, PJ
通讯作者：
RANDALL, PJ
影响因子：
7.4
作者：
DELHAIZE, E;RYAN, PR
通讯作者：
RYAN, PR
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
4.9
作者：
Delhaize, E;Ryan, PR;Richardson, AE
通讯作者：
Richardson, AE