Helper activity of CD4+ αβ T cells is required for the avian γδ T cell response
Helper activity of CD4+ αβ T cells is required for the avian γδ T cell response
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CD4+ αβ T 细胞的辅助活性是鸟类 γδ T 细胞反应所必需的
DOI：
10.1002/eji.1830230848
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发表时间：
1993
影响因子：
5.4
通讯作者：
O. Lassila
中科院分区：
文献类型：
--
作者：
T. Arstila;P. Toivanen;O. Lassila
我们研究了鸡γ δ T细胞的体外活化。从完全弗氏佐剂致敏的鸡获得的脾α β和γ δ T细胞在用分枝杆菌超声处理物或结核分枝杆菌的纯化蛋白衍生物刺激时在体外增殖。当去除CD4+细胞或α β T细胞受体（TcR）阳性细胞时，γ δ T细胞响应分枝杆菌抗原的增殖和原始细胞形成均被消除。如果将伴刀豆球蛋白A（Con A）激活的淋巴细胞培养物（CAS）的上清液作为辅助因子的来源与特异性抗原纯化蛋白衍生物一起加入，则应答恢复。CD4或α β TcR耗竭的细胞对Con A仍有增殖反应，但观察到反应降低。为了更特异地分析γ δ T细胞应答，我们用针对T细胞受体的固定化单克隆抗体刺激外周血细胞。单独的抗γ δ TcR抗体不诱导显著增殖。当CAS与抗γ δ TcR单克隆抗体一起加入时，观察到γ δ T细胞的强烈增殖。相比之下，表达V β 1和V β 2的α β T细胞在体外对单独使用相关抗TcR单克隆抗体刺激的反应中增殖。单独消耗V β 1+或V β 2 + T细胞亚群对分枝杆菌抗原刺激的γ δ T细胞的增殖或原始细胞形成没有负面影响。综上所述，我们的结果表明，CD4 + α β T细胞（V β 1-和V β 2-表达）在鸡γ δ T细胞的活化和应答中起作用。
We have studied the in vitro activation of chicken γδ T cells. Both splenic αβ and γδ T cells obtained from complete Freund's adjuvant‐primed chickens proliferated in vitro when stimulated with mycobacterial sonicate or purified protein derivative of Mycobacterium tuberculosis. When CD4+ cells or αβ T cell receptor (TcR)‐positive cells were removed, both the proliferation and the blast formation of γδ T cells in response to mycobacterial antigens were abrogated. The response was restored if supernatant from concanavalin A (Con A)‐activated lymphocyte cultures (CAS) as a source of helper factors was added together with the specific antigen purified protein derivative. The CD4‐ or αβ TcR‐depleted cells still proliferated in response to Con A, although a decrease of the response was observed. To analyze the γδ T cell response more specifically we stimulated peripheral blood cells with immobilized monoclonal antibodies against T cell receptor. Anti‐γδ TcR antibody alone did not induce significant proliferation. When CAS was added together with the anti‐γδ TcR monoclonal antibody, a strong proliferation of γδ T cells was observed. In contrast, both Vβ1‐ and Vβ2‐expressing αβ T cells proliferated in vitro in response to stimulation with the relevant anti‐TcR monoclonal antibody alone. Depletion of either Vβ1+ or Vβ2+ T cell subset alone had no negative effect on the proliferation or blast formation of γδ T cells stimulated with mycobacterial antigens. Taken together our results suggest that CD4+ αβ T cells (both Vβl‐ and Vβ2‐expressing) play a role in the activation and response of chicken γδ T cells.
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影响因子：
4.4
作者：
R. P. Bucy;C.-L. H. Chen;J. Cihak;U. Lösch;M D Cooper
通讯作者：
R. P. Bucy;C.-L. H. Chen;J. Cihak;U. Lösch;M D Cooper
CD4 细胞在控制细胞毒性 T 淋巴细胞生成中的两个作用。
DOI：
--
发表时间：
1991
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Cassell,D;Forman,J
通讯作者：
Forman,J
禽T细胞个体发育。
影响因子：
--
作者：
Cooper,MD;Chen,CL;Bucy,RP;Thompson,CB
通讯作者：
Thompson,CB
禽类 T 细胞的第三个亚系可以用 T 细胞受体 3 特异性抗体来鉴定。
DOI：
--
发表时间：
1990
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Char,D;Sanchez,P;Chen,CL;Bucy,RP;Cooper,MD
通讯作者：
Cooper,MD