Prion-Like Domains in Phagobiota
Prion-Like Domains in Phagobiota
复制标题
噬菌体中的朊病毒样结构域
作者：
G. Tetz;V. Tetz
朊病毒是一种具有自我繁殖特性的分子，它可以通过构象转换产生新的朊病毒。朊病毒蛋白最初与哺乳动物病理学的发展有关;然而，最近它们已被证明有助于各种原核生物和真核生物的环境适应。噬菌体是广泛存在的，代表了微生物群稳态的重要调节剂，并且已经显示出在各种细菌家族中是多样的。在这里，我们研究了噬菌体是否含有朊病毒样蛋白，以及这些朊病毒样蛋白结构域是否参与体内平衡的调节。我们使用了一种计算算法，朊病毒样氨基酸组成，检测朊病毒样结构域在370,617公开可获得的噬菌体蛋白质序列，这导致在5040推定的朊病毒的鉴定。我们分析了一组朊病毒样蛋白，并观察到它们在不同噬菌体家族中的分布，与它们与细菌宿主细胞的相互作用有关。我们发现朊病毒样结构域可以在各种细菌和古细菌中找到。本研究的结果表明，噬菌体朊病毒样蛋白主要参与噬菌体与细菌细胞之间的相互作用，例如与噬菌体在细胞中的附着和穿透以及噬菌体后代的释放相关的蛋白。这些数据允许识别噬菌体朊病毒样蛋白作为噬菌体和细菌细胞之间相互作用的新调节剂。
Prions are molecules characterized by self-propagation, which can undergo a conformational switch leading to the creation of new prions. Prion proteins have originally been associated with the development of mammalian pathologies; however, recently they have been shown to contribute to the environmental adaptation in a variety of prokaryotic and eukaryotic organisms. Bacteriophages are widespread and represent the important regulators of microbiota homeostasis and have been shown to be diverse across various bacterial families. Here, we examined whether bacteriophages contain prion-like proteins and whether these prion-like protein domains are involved in the regulation of homeostasis. We used a computational algorithm, prion-like amino acid composition, to detect prion-like domains in 370,617 publicly available bacteriophage protein sequences, which resulted in the identification of 5040 putative prions. We analyzed a set of these prion-like proteins, and observed regularities in their distribution across different phage families, associated with their interactions with the bacterial host cells. We found that prion-like domains could be found across all phages of various groups of bacteria and archaea. The results obtained in this study indicate that bacteriophage prion-like proteins are predominantly involved in the interactions between bacteriophages and bacterial cell, such as those associated with the attachment and penetration of bacteriophage in the cell, and the release of the phage progeny. These data allow the identification of phage prion-like proteins as novel regulators of the interactions between bacteriophages and bacterial cells.
参与尾部完成的噬菌体 P2 基因 R 和 S 的分子克隆和表征。
影响因子：
3.7
作者：
Linderoth,NA;Julien,B;Flick,KE;Calendar,R;Christie,GE
通讯作者：
Christie,GE
影响因子：
56.9
作者：
BOLTON, DC;MCKINLEY, MP;PRUSINER, SB
通讯作者：
PRUSINER, SB
影响因子：
15.9
作者：
Blanco, Luz P.;Evans, Margery L.;Smith, Daniel R.;Badtke, Matthew P.;Chapman, Matthew R.
通讯作者：
Chapman, Matthew R.