Complete genome sequence of a serotype 11A, ST62 Streptococcus pneumoniae invasive isolate.
Complete genome sequence of a serotype 11A, ST62 Streptococcus pneumoniae invasive isolate.
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DOI：
10.1186/1471-2180-11-25
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发表时间：
2011-02-01
期刊：
影响因子：
4.2
通讯作者：
Pantosti A
中科院分区：
文献类型：
--
作者：
Camilli R;Bonnal RJ;Del Grosso M;Iacono M;Corti G;Rizzi E;Marchetti M;Mulas L;Iannelli F;Superti F;Oggioni MR;De Bellis G;Pantosti A
肺炎链球菌是一种重要的人类病原体，是世界范围内发病率和死亡率的主要原因。我们对一株由于erm(Tr)决定簇的存在而对红霉素耐药的11A型肺炎链球菌侵袭性分离株(AP200)的基因组进行了测序，并对基因组结构进行了分析，并与其他肺炎球菌基因组进行了比较。肺炎链球菌AP200基因组序列全长2,130,580个碱基对。基因组包含2216个编码序列(CDS)、56个tRNA和12个rRNA基因。在CDS中，72.9%具有预测的生物学已知功能。AP200含有毛状胰岛2，虽然其表型对应于11A血清型，但它含有一个11D囊位。观察到由复制轴上的大倒置引起的染色体重排和水平基因转移事件。染色体倒位可能与两个大的外源元件的插入影响染色体结构的重新平衡有关，这两个外源元件是携带Erm(Tr)的Tn1806和一个被命名为ϕSpn_200的功能原噬菌体。Tn1806全长52,457bp，由49个开放阅读框组成。对Tn1806的比较分析表明，在相关物种中，如化脓性链球菌，以及在厌氧物种Finegoldia Magna、厌氧菌和艰难梭菌中，也存在类似的遗传元件或部分遗传元件。ϕSpN_200基因组全长35,989个碱基，由47个开放阅读框组成，分为5个功能模块。在肺炎球菌和其他链球菌中发现了与ϕSpn_200类似的前驱噬菌体，显示出高度的功能模块交换。ϕSpn_200病毒颗粒具有典型的Sphopviridae家族的形态特征，能够感染肺炎球菌受体株。肺炎链球菌AP200染色体序列显示了一个动态的基因组，具有染色体重排和水平基因转移的特点。AP200的整体多样性主要是由外源元件Tn1806和ϕSpn_200的存在驱动的，它们与不同细菌物种的其他遗传元件进行了大量的基因交换。这些遗传因素可能会为AP200提供额外的基因，例如那些赋予抗生素抗药性的基因，促进其对环境的适应。
Streptococcus pneumoniae is an important human pathogen representing a major cause of morbidity and mortality worldwide. We sequenced the genome of a serotype 11A, ST62 S. pneumoniae invasive isolate (AP200), that was erythromycin-resistant due to the presence of the erm(TR) determinant, and carried out analysis of the genome organization and comparison with other pneumococcal genomes. The genome sequence of S. pneumoniae AP200 is 2,130,580 base pair in length. The genome carries 2216 coding sequences (CDS), 56 tRNA, and 12 rRNA genes. Of the CDSs, 72.9% have a predicted biological known function. AP200 contains the pilus islet 2 and, although its phenotype corresponds to serotype 11A, it contains an 11D capsular locus. Chromosomal rearrangements resulting from a large inversion across the replication axis, and horizontal gene transfer events were observed. The chromosomal inversion is likely implicated in the rebalance of the chromosomal architecture affected by the insertions of two large exogenous elements, the erm(TR)-carrying Tn1806 and a functional prophage designated ϕSpn_200. Tn1806 is 52,457 bp in size and comprises 49 ORFs. Comparative analysis of Tn1806 revealed the presence of a similar genetic element or part of it in related species such as Streptococcus pyogenes and also in the anaerobic species Finegoldia magna, Anaerococcus prevotii and Clostridium difficile. The genome of ϕSpn_200 is 35,989 bp in size and is organized in 47 ORFs grouped into five functional modules. Prophages similar to ϕSpn_200 were found in pneumococci and in other streptococcal species, showing a high degree of exchange of functional modules. ϕSpn_200 viral particles have morphologic characteristics typical of the Siphoviridae family and are capable of infecting a pneumococcal recipient strain. The sequence of S. pneumoniae AP200 chromosome revealed a dynamic genome, characterized by chromosomal rearrangements and horizontal gene transfers. The overall diversity of AP200 is driven mainly by the presence of the exogenous elements Tn1806 and ϕSpn_200 that show large gene exchanges with other genetic elements of different bacterial species. These genetic elements likely provide AP200 with additional genes, such as those conferring antibiotic-resistance, promoting its adaptation to the environment.
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影响因子：
3.7
作者：
Beres SB;Musser JM
通讯作者：
Musser JM
影响因子：
4.9
作者：
Carnilli, Rornina;Del Grosso, Maria;Pantostil, Annalisa
通讯作者：
Pantostil, Annalisa
影响因子：
3.2
作者：
Bagnoli, Fabio;Moschioni, Monica;Barocchi, Michele A.
通讯作者：
Barocchi, Michele A.
DOI：
10.1073/pnas.0511017103
发表时间：
2006-02-21
影响因子：
11.1
作者：
Barocchi, MA;Rie, J;Henriques-Normark, B
通讯作者：
Henriques-Normark, B
影响因子：
8
作者：
Huang SS;Hinrichsen VL;Stevenson AE;Rifas-Shiman SL;Kleinman K;Pelton SI;Lipsitch M;Hanage WP;Lee GM;Finkelstein JA
通讯作者：
Finkelstein JA