Self-sustained replication of an RNA enzyme.
Self-sustained replication of an RNA enzyme.
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DOI：
10.1126/science.1167856
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发表时间：
2009-02-27
期刊：
影响因子：
--
通讯作者：
Joyce GF
中科院分区：
文献类型：
--
作者：
Lincoln TA;Joyce GF
RNA酶已经在没有蛋白质的情况下进行了自我维持的复制，为人工遗传系统提供了基础。一种催化RNA模板连接RNA的RNA酶被转化为两种酶相互催化对方合成的形式，总共有四种组成底物。这些交叉复制的RNA酶经过优化，使它们可以在恒定温度下，在没有蛋白质或其他生物材料的情况下进行自我维持的指数扩增。扩增发生时需要大约一个小时的加倍时间，并且可以无限期地持续下去。构建了各种交叉复制酶的种群，并允许它们竞争共同的底物池。在一系列转移实验中，种群经历了1025倍的整体扩增，重组复制子出现并增长，并在种群中占据主导地位。经过自我维持复制的RNA酶可以作为遗传系统的实验模型。可以构建许多这样的模型系统，允许不同的选择性结果与遗传系统的基本属性相关。
RNA enzymes have been made to undergo self-sustained replication in the absence of proteins, providing the basis for an artificial genetic system. An RNA enzyme that catalyzes the RNA-templated joining of RNA was converted to a format whereby two enzymes catalyze each other’s synthesis from a total of four component substrates. These cross-replicating RNA enzymes were optimized so that they can undergo self-sustained exponential amplification at a constant temperature and in the absence of proteins or other biological materials. Amplification occurs with a doubling time of about one hour, and can be continued indefinitely. Populations of various cross-replicating enzymes were constructed and allowed to compete for a common pool of substrates. During a serial transfer experiment in which the population underwent overall amplification of >1025-fold, recombinant replicators arose and grew to dominate the population. RNA enzymes that undergo self-sustained replication can serve as an experimental model of a genetic system. Many such model systems could be constructed, allowing different selective outcomes to be related to the underlying properties of the genetic system.
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影响因子：
5.6
作者：
CRICK, FHC
通讯作者：
CRICK, FHC
DOI：
10.1073/pnas.202471099
发表时间：
2002-10-01
影响因子：
11.1
作者：
Paul, N;Joyce, GF
通讯作者：
Joyce, GF
影响因子：
--
作者：
Kim, DE;Joyce, GF
通讯作者：
Joyce, GF
影响因子：
64.8
作者：
Szostak, JW;Bartel, DP;Luisi, PL
通讯作者：
Luisi, PL
影响因子：
3.5
作者：
Kim, Ki-Sun;Oh, Sangtaek;Kim, Dong-Eun
通讯作者：
Kim, Dong-Eun