The hormonal coordination of cuticulin deposition and morphogenesis in Drosophila imaginal discs in vivo and in vitro.
The hormonal coordination of cuticulin deposition and morphogenesis in Drosophila imaginal discs in vivo and in vitro.
复制标题
体内和体外果蝇成虫盘角质素沉积和形态发生的激素协调。
DOI：
10.1016/0012-1606(86)90378-7
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发表时间：
1986
影响因子：
2.7
通讯作者：
W. Liebrich
中科院分区：
文献类型：
--
作者：
D. Fristrom;W. Liebrich
角质蛋白是昆虫角质层的第一层，它以连续的非弹性层的形式覆盖在角质分泌细胞的顶面上。在果蝇变态过程中，蛹形成后3 ~ 7小时，成虫盘存款在蛹表皮的角质层上。这是一个快速形态发生的时期，涉及细胞形状变化和细胞重排。为了了解表皮蛋白沉积与形态发生的协调关系，我们在体内和体外研究了表皮蛋白沉积。我们发现，最佳的激素方案（类固醇激素，20-羟基蜕皮激素）完成形态发生和角质沉积在vitroparallel在体内激素滴度的变化。我们还发现，角质层沉积最后超过细胞边界，从而使细胞重排发生角质层奠定了。我们已经确定在体外条件下，角质蛋白沉积完成早熟，抑制进一步的形态发生。细胞松弛素B和秋水仙碱不抑制角质蛋白沉积，因此我们得出结论，一个完整的细胞骨架是不必要的分泌这种细胞外结构。最后，我们提出了一个初步的协议，部分纯化角质素合成在vitroby质量分离盘。
Cuticulin is the first layer of the insect cuticle to be deposited and is laid down as a continuous inelastic sheet over the apical surface of cuticle-secreting cells. During metamorphosis inDrosophila melanogaster, imaginal discs deposit the cuticulin layer of the pupal cuticle between 3 and 7 hr after puparium formation. This is a period of rapid morphogenesis involving cell shape changes and cell rearrangements. We have examined cuticulin depositionin vivoandin vitrowith a view to understanding the coordination of cuticulin deposition with morphogenesis. We find that the optimum hormonal regimen (of the steroid hormone, 20-hydroxyecdysone) for the completion of both morphogenesis and cuticulin depositionin vitroparallels the changes in hormone titer observedin vivo. We also find that cuticulin is deposited last over cell boundaries, thereby allowing cell rearrangements to occur as cuticulin is laid down. We have identifiedin vitroconditions under which cuticulin deposition is completed precociously, inhibiting further morphogenesis. Cytochalasin B and colchicine do not inhibit cuticulin deposition and we therefore conclude that an intact cytoskeleton is not necessary for secretion of this extracellular structure. Finally, we present a preliminary protocol for the partial purification of cuticulin synthesizedin vitroby mass isolated discs.
影响因子：
2.7
作者：
L. Schwartz;J. Truman
通讯作者：
L. Schwartz;J. Truman