Fluorescent lectin staining of Drosophila embryos and tissues to detect the spatial distribution of glycans during development.

Fluorescent lectin staining of Drosophila embryos and tissues to detect the spatial distribution of glycans during development.
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果蝇胚胎和组织的荧光凝集素染色可检测发育过程中聚糖的空间分布。

DOI:
10.1007/978-1-62703-465-4_8
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发表时间:
2013
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
TenHagen,KellyG
TenHagen,KellyG
中科院分区:
--
文献类型:
--
作者:
Tian,E;Zhang,Liping;TenHagen,KellyG

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Glycans are the result of the coordinated activities of glycosyltransferases responsible for specific sugar additions. Glycans present on proteins can influence protein stability, transport, function, and recognition, and thus can have profound effects on cell–cell interactions, adhesion, and signaling events occurring during eukaryotic development. Lectin staining provides a useful tool to detect the spatial distribution of specific glycans in developing tissues in situ. Here we describe a method to detect diverse glycans present in developingDrosophilatissues and organs using fluorescently labeled lectins.
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