Extensive intra-kingdom horizontal gene transfer converging on a fungal fructose transporter gene.
Extensive intra-kingdom horizontal gene transfer converging on a fungal fructose transporter gene.
复制标题
DOI：
10.1371/journal.pgen.1003587
复制
发表时间：
2013-06
期刊：
影响因子：
4.5
通讯作者：
Gonçalves P
中科院分区：
文献类型：
--
作者：
Coelho MA;Gonçalves C;Sampaio JP;Gonçalves P
比较基因组学在过去十年中揭示了原核生物之间猖獗的水平基因转移（HGT）的情景，但对于真菌来说，垂直遗传的明显主导模式仍然存在，但被越来越多的例外打断。在本工作中，我们研究了真菌基因编码的果糖转运蛋白（FSY 1）具有独特的底物选择性的系统发育分布和遗传模式。在一项包括241个可用真菌基因组的调查中，在子囊菌门的两个亚门中鉴定了109个FSY1同源物。至少有10个独立的物种间的水平基因转移（HGT）的情况下，涉及FSY1的确定，支持强有力的系统发育证据和同线性分析。通过HGT获得FSY 1有时提示异种基因置换，但也发现了几例假旁系同源。此外，发现了连续HGT事件的证据，可能包括那些负责在酵母谱系中传递基因的事件。这些事件似乎并不驱动Fsy1蛋白的功能多样化，因为Fsy1同源物从遥远的血统，包括至少一个收购HGT，似乎有类似的生化特性。总之，追溯FSY1基因的进化路径揭示了涉及单个真菌基因的空前数量的独立HGT事件。我们认为，该基因的动荡的进化历史可能与编码的转运蛋白的独特的生化特性，其可预测的健身效果可能是高度可变的。总的来说，我们的研究结果支持了最近的观点，表明种间HGT可能比迄今为止假设的对真菌基因组的形成有更大的贡献。基因通常是垂直遗传的，这意味着它们与发现它们的生物体具有相同的进化历史。然而，它们也可以在具有重叠生态位的物种之间传递，这种现象称为水平基因转移（HGT），可以发生在密切相关的物种之间，也可以发生在属于不同生命领域的生物体之间。虽然HGT在原核生物中非常常见，但在真核生物（包括真核微生物）中的报道较少。在真菌中，HGT从细菌获得基因的几个例子已经报道，但真菌物种之间的基因交换被认为是罕见的。在这里，我们描述了我们的发现，关于一个单一的真菌基因，似乎已经转移真菌之间非常频繁。我们认为这可能与这样一个事实有关，即该基因对宿主既非常有用，又有害，这取决于遗传背景和环境。我们的研究结果表明，真菌之间的基因交换可能比迄今为止所假设的要频繁得多。
Comparative genomics revealed in the last decade a scenario of rampant horizontal gene transfer (HGT) among prokaryotes, but for fungi a clearly dominant pattern of vertical inheritance still stands, punctuated however by an increasing number of exceptions. In the present work, we studied the phylogenetic distribution and pattern of inheritance of a fungal gene encoding a fructose transporter (FSY1) with unique substrate selectivity. 109 FSY1 homologues were identified in two sub-phyla of the Ascomycota, in a survey that included 241 available fungal genomes. At least 10 independent inter-species instances of horizontal gene transfer (HGT) involving FSY1 were identified, supported by strong phylogenetic evidence and synteny analyses. The acquisition of FSY1 through HGT was sometimes suggestive of xenolog gene displacement, but several cases of pseudoparalogy were also uncovered. Moreover, evidence was found for successive HGT events, possibly including those responsible for transmission of the gene among yeast lineages. These occurrences do not seem to be driven by functional diversification of the Fsy1 proteins because Fsy1 homologues from widely distant lineages, including at least one acquired by HGT, appear to have similar biochemical properties. In summary, retracing the evolutionary path of the FSY1 gene brought to light an unparalleled number of independent HGT events involving a single fungal gene. We propose that the turbulent evolutionary history of the gene may be linked to the unique biochemical properties of the encoded transporter, whose predictable effect on fitness may be highly variable. In general, our results support the most recent views suggesting that inter-species HGT may have contributed much more substantially to shape fungal genomes than heretofore assumed. Genes are commonly vertically inherited, meaning that they share the evolutionary history of the organisms in which they are found. However, they can also be transmitted between species with overlapping niches, a phenomenon known as horizontal gene transfer (HGT) that can occur between closely related species but also between organisms belonging to different domains of life. While HGT is very common in prokaryotes, it has been less frequently reported in eukaryotes, including eukaryotic microbes. In fungi, several instances of genes acquired by HGT from bacteria have been reported, but gene exchange between fungal species is thought to be rare. Here, we describe our findings concerning a single fungal gene that seems to have been transferred between fungi very often. We believe this may be related to the fact that the gene can be both very useful and detrimental for the host, depending on genetic background and environment. Our results suggest that exchange of genes between fungi may happen much more frequently than assumed so far.
登录
查看更多内容
影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
影响因子：
4.4
作者：
Fitzpatrick, David A.;O'Gaora, Peadar;Butler, Geraldine
通讯作者：
Butler, Geraldine
影响因子：
10.7
作者：
Chow, Eve W. L.;Morrow, Carl A.;Fraser, James A.
通讯作者：
Fraser, James A.
影响因子：
10.7
作者：
Bryant, D;Moulton, V
通讯作者：
Moulton, V
影响因子：
30.8
作者：
Friesen, Timothy L.;Stukenbrock, Eva H.;Oliver, Richard P.
通讯作者：
Oliver, Richard P.