TMK-based cell-surface auxin signalling activates cell-wall acidification.
TMK-based cell-surface auxin signalling activates cell-wall acidification.
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DOI：
10.1038/s41586-021-03976-4
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发表时间：
2021-11
期刊：
影响因子：
64.8
通讯作者：
Yang Z
中科院分区：
文献类型：
--
作者：
Lin W;Zhou X;Tang W;Takahashi K;Pan X;Dai J;Ren H;Zhu X;Pan S;Zheng H;Gray WM;Xu T;Kinoshita T;Yang Z
植物激素生长素控制着植物的许多过程，至少部分是通过它对细胞扩张的调节。酸生长假说已经被提出来解释生长素刺激的细胞扩张的五十年，但生长素诱导的细胞壁酸化的机制是很差的特点。生长素诱导质膜H+-ATP酶的磷酸化和激活，该酶将质子泵入质外体，但生长素如何激活其磷酸化仍不清楚。在这里，我们表明，跨膜激酶（TMK）生长素信号蛋白与质膜H+-ATP酶相互作用，诱导其磷酸化，从而促进细胞壁酸化和下胚轴细胞伸长拟南芥。生长素诱导TMKs和H+-ATP酶之间的相互作用在质膜上的几秒钟内，以及TMK依赖的磷酸化的倒数第二个苏氨酸残基的H+-ATP酶。我们的遗传学、生物化学和分子生物学证据表明，TMKs直接磷酸化质膜H+-ATP酶，并且是生长素诱导的H+-ATP酶激活、质外体酸化和细胞扩增所必需的。因此，我们的研究结果揭示了生长素和质膜H+-ATP酶激活调节质外体pH值的变化和细胞扩张，通过TMK为基础的细胞表面生长素信号之间的重要联系。生长素通过其倒数第二个苏氨酸残基的磷酸化诱导质膜H+-ATP酶的跨膜激酶依赖性激活，促进拟南芥质外体酸化和下胚轴细胞伸长。
The phytohormone auxin controls many processes in plants, at least in part through its regulation of cell expansion. The acid growth hypothesis has been proposed to explain auxin-stimulated cell expansion for five decades, but the mechanism that underlies auxin-induced cell-wall acidification is poorly characterized. Auxin induces the phosphorylation and activation of the plasma membrane H+-ATPase that pumps protons into the apoplast, yet how auxin activates its phosphorylation remains unclear. Here we show that the transmembrane kinase (TMK) auxin-signalling proteins interact with plasma membrane H+-ATPases, inducing their phosphorylation, and thereby promoting cell-wall acidification and hypocotyl cell elongation in Arabidopsis. Auxin induced interactions between TMKs and H+-ATPases in the plasma membrane within seconds, as well as TMK-dependent phosphorylation of the penultimate threonine residue on the H+-ATPases. Our genetic, biochemical and molecular evidence demonstrates that TMKs directly phosphorylate plasma membrane H+-ATPase and are required for auxin-induced H+-ATPase activation, apoplastic acidification and cell expansion. Thus, our findings reveal a crucial connection between auxin and plasma membrane H+-ATPase activation in regulating apoplastic pH changes and cell expansion through TMK-based cell surface auxin signalling. Auxin induces transmembrane-kinase-dependent activation of H+-ATPase in the plasma membrane through phosphorylation of its penultimate threonine residue, promoting apoplastic acidification and hypocotyl cell elongation in Arabidopsis.
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DOI：
10.1073/pnas.1613499114
发表时间：
2017-06-13
影响因子：
11.1
作者：
Barbez, Elke;Duenser, Kai;Busch, Wolfgang
通讯作者：
Busch, Wolfgang
影响因子：
64.8
作者：
Cao, Min;Chen, Rong;Xu, Tongda
通讯作者：
Xu, Tongda
DOI：
10.1016/j.cub.2009.08.052
发表时间：
2009-11-17
期刊：
Current biology : CB
影响因子：
--
作者：
Fu Y;Xu T;Zhu L;Wen M;Yang Z
通讯作者：
Yang Z
影响因子：
3.7
作者：
Dai N;Wang W;Patterson SE;Bleecker AB
通讯作者：
Bleecker AB
影响因子：
4.8
作者：
Fuglsang, AT;Visconti, S;Palmgren, MG
通讯作者：
Palmgren, MG