Immunogenicity of a synthetic oligopeptide corresponding to antigenically common T-helper and B-cell neutralizing epitopes of the major outer membrane protein of Chlamydia trachomatis.
Immunogenicity of a synthetic oligopeptide corresponding to antigenically common T-helper and B-cell neutralizing epitopes of the major outer membrane protein of Chlamydia trachomatis.
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与沙眼衣原体主要外膜蛋白的抗原性常见 T 辅助细胞和 B 细胞中和表位相对应的合成寡肽的免疫原性。
DOI：
10.1016/0264-410x(93)90080-h
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发表时间：
1993
期刊：
影响因子：
5.5
通讯作者：
H. Caldwell
中科院分区：
文献类型：
--
作者：
H. Su;H. Caldwell
由沙眼衣原体引起的性传播疾病（STD）是一个重要的公共卫生问题，迫切需要预防或控制这些疾病的疫苗。主要外膜蛋白（MOMP）是开发衣原体性传播疾病亚单位疫苗的主要候选抗原。合成寡肽（称为 A8-VDIV）的免疫原性，对应于含有 C. 和 C. 两者的 MOMP 序列。在小鼠和非人灵长类动物中研究了沙眼种常见 T 辅助细胞 (A8) 和 B 细胞 (VDIV) 表位。用肽 A8-VDIV 免疫的 8 个 H-2 同源小鼠品系中的 6 个产生了针对寡肽的 VDIV B 细胞部分的高滴度 IgG 抗体。通过 pepscan ELISA 对抗肽抗体的精细图谱显示，每种响应的小鼠品系都产生与 VDIV 序列中包含的物种常见七聚体中和表位 298LNPTIAG304 反应的抗体。小鼠抗肽抗体与完整C发生反应。通过 ELISA 检测沙眼原发性小体 (EB)，并中和具有亚种特异性的培养真核细胞的衣原体感染性。用肽 A8-VDIV 免疫三只食蟹猴，并类似地研究了它们的 IgG 抗体反应。所有三只猴子都产生了 IgG 抗体，该抗体与 VDIV 肽发生反应，并识别 VDIV 序列内物种共有的 LNPTIAG 中和位点。猴抗肽抗体与完整C结合。沙眼EBs并在体外被中和。肽 A8-VDIV 在不同品系小鼠中的免疫原性在 H-2 处有所不同，其在非人灵长类动物中的免疫原性及其针对多重 C 的交叉反应性中和抗体应答的能力也不同。就寡肽作为针对衣原体 STD 的实验性疫苗的潜在用途而言，沙眼病毒变种是令人鼓舞的发现。
Sexually transmitted diseases (STDs) caused byChlamydia trachomatisare an important public health problem and a vaccine to prevent or control these diseases is badly needed. The major outer membrane protein (MOMP) is the principal candidate antigen for the development of subunit vaccine against chlamydial STDs. The immunogenicity of a synthetic oligopeptide, termed A8-VDIV, corresponding to MOMP sequences containing bothC. trachomatisspecies common T-helper (A8) and B-cell (VDIV) epitopes was studied in mice and non-human primates. Six of eight H-2 congenic mouse strains immunized with peptide A8-VDIV produced high-titre IgG antibodies against the VDIV B-cell portion of the oligopeptide. Fine mapping of the anti-peptide antibodies by pepscan ELISA showed that each of the responding mouse strains made antibodies reactive with a species-common septmeric neutralizing epitope298LNPTIAG304contained in the VDIV sequence. The mouse anti-peptide antibodies reacted with intactC. trachomatiselementary bodies (EBs) by ELISA and neutralized chlamydial infectivity for cultured eukaryotic cells with sub-species specificity. Three cynomolgus monkeys were immunized with peptide A8-VDIV and their IgG antibody responses were similarly studied. All three monkeys produced IgG antibodies which reacted with the VDIV peptide and which recognized the species-common LNPTIAG neutralizing site within the VDIV sequence. Monkey anti-peptide antibodies bound to intactC. trachomatisEBs and were neutralizingin vitro. The immunogenicity of peptide A8-VDIV in different strains of mice disparate at H-2, its immunogenicity in non-human primates, and its ability to target cross-reactive neutralizing antibody responses against multipleC. trachomatisserovars are encouraging findings in terms of the potential utility of the oligopeptide as an experimental vaccine against chlamydial STDs.
用单克隆抗体对沙眼衣原体进行免疫分型。
DOI：
10.1093/infdis/152.4.791
发表时间：
1985
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Wang,SP;Kuo,CC;Barnes,RC;Stephens,RS;Grayston,JT
通讯作者：
Grayston,JT
保护性单克隆抗体可识别位于沙眼衣原体主要外膜蛋白上的表位。
DOI：
--
发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Zhang,YX;Stewart,S;Joseph,T;Taylor,HR;Caldwell,HD
通讯作者：
Caldwell,HD