Infrared Spectroscopic Imaging Advances as an Analytical Technology for Biomedical Sciences.
Infrared Spectroscopic Imaging Advances as an Analytical Technology for Biomedical Sciences.
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DOI：
10.1021/acs.analchem.7b05330
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发表时间：
2018-02-06
影响因子：
7.4
通讯作者：
Bhargava R
中科院分区：
文献类型：
--
作者：
Wrobel TP;Bhargava R
光学化学成像试图在不使用标记或探针的情况下非破坏性地获取空间分辨的化学信息。振动中红外（IR）光谱和显微镜的组合是特别有吸引力的，因为样品的基本振动模式与光学频率一致，从而吸收了大部分入射光，并提供了强信号。现代红外光谱成像可以追溯到近25年前，将红外显微镜耦合到阵列探测器和干涉仪。近二十年来，仪器在很大程度上类似于这种初始设置，具有几项创新，如快速扫描成像，时间分辨成像，更快的探测器和线性阵列系统。6这些进步提供了几种变体，以加速数据采集，并与宽带球光源，干涉仪和阵列检测装备显微镜的基本配置相比，实现新的功能。在过去的几年里，硬件和设计的最新进展极大地改变了仪器和可用性。新组件的可用性导致了仪器的多样性;通过严格的理论对图像形成的新理解导致了新的设计，因此，新的应用带来了显着的进步。分析测量能力的巨大扩展和令人兴奋的新可能性现在已经变得显而易见。在这里，我们回顾这些进展，并组织各种发展的框架内转换的分析性能的红外成像的空间，时间和信息内容。一些优秀的评论涵盖了该技术的基础知识，7仪器，8，9和应用9 - 11以及在聚合物中的应用，12，13植物生物学，14制药，爆炸物，法医应用，15和生物医学成像方面。16− 18在仪器方面，大多数评论自然集中在使用阵列探测器的傅里叶变换红外（FT-IR）成像技术上。简而言之，最先进的FTIR成像系统由具有焦平面阵列（FPA）检测器的显微镜（图1）组成。最常见的探测器材料是碲镉汞（MCT），
Optical chemical imaging seeks to nondestructively acquire spatially resolved chemical information without the use of labels or probes. The combination of vibrational mid-infrared (IR) spectroscopy and microscopy is especially attractive since the fundamental vibrational modes of samples are coincident with optical frequencies, thereby absorbing a large fraction of incident light and providing a strong signal. Modern IR spectroscopic imaging can be traced back nearly 25 years, with the coupling of an IR microscope to an array detector and an interferometer. 1, 2 Instrumentation had been largely similar to this initial setup for almost two decades with several innovations such as rapid scan imaging, 3, 4 time-resolved imaging, 5 faster detectors, and linear array systems. 6 These advances have provided several variants to speed up data acquisition and enable new capabilities compared to the basic configuration of a broadband globar source, interferometer, and array detectorequipped microscope. Recent advances in hardware and design have dramatically changed both instrumentation and availability over the past few years. The availability of new components has led to a diversity in instrumentation; new understanding of image formation by rigorous theory has led to new designs and, consequently, novel applications have resulted in notable progress. A tremendous expansion in capability and exciting new possibilities for analytical measurements have now become apparent. Here, we review these advances and organize the various developments in the framework of transforming the analytical performance of IR imaging in terms of spatial, temporal, and information content. Several excellent reviews cover the fundamentals of the technology, 7 instrumentation, 8, 9 and applications 9− 11 as well as applications in polymers, 12, 13 plant biology, 14 pharmaceuticals, explosives, forensic applications, 15 and aspects of biomedical imaging. 16− 18 In terms of instrumentation, most reviews naturally focus on Fourier transform IR (FT-IR) imaging technology using array detectors. Briefly, a state-of-the-art FTIR imaging system consists of a microscope (Figure 1) with a focal plane array (FPA) detector. The most common detector material is mercury− cadmium− telluride (MCT) to enable wide
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影响因子：
3.5
作者：
Bhargava, R;Levin, IW
通讯作者：
Levin, IW
影响因子：
4.2
作者：
Berisha, Sebastian;Chang, Shengyuan;Mayerich, David
通讯作者：
Mayerich, David
影响因子：
6.1
作者：
Birarda, G.;Ravasio, A.;Grenci, G.
通讯作者：
Grenci, G.
影响因子：
4.2
作者：
Barlow, Daniel E.;Biffinger, Justin C.;Russell, John N., Jr.
通讯作者：
Russell, John N., Jr.
影响因子：
7.4
作者：
Bassan, Paul;Mellor, Joe;Gardner, Peter
通讯作者：
Gardner, Peter