Metabolic Engineering of Microorganisms for the Production of Flavonoids.
Metabolic Engineering of Microorganisms for the Production of Flavonoids.
复制标题
微生物代谢工程用于生产黄酮。
DOI：
10.3389/fbioe.2020.589069
复制
发表时间：
2020
影响因子：
5.7
通讯作者：
Shen X
中科院分区：
文献类型：
--
作者：
Sheng H;Sun X;Yan Y;Yuan Q;Wang J;Shen X
黄酮类化合物是一类存在于植物和真菌中的次生代谢产物。由于其具有抗衰老、抗氧化、抗炎、抗癌等重要的生物活性，被广泛应用于食品、医药和保健品行业。传统的黄酮类化合物的生产方法包括化学合成法和植物提取法，原料危险，工艺复杂，产品效价低，收率低。利用可再生生物质如葡萄糖和木糖合成黄酮类化合物被认为是一种可持续和环境友好的大规模生产黄酮类化合物的方法。近年来，构建高效生物合成黄酮类化合物的微生物细胞工厂受到广泛关注。本文综述了近年来微生物合成黄酮类化合物的研究进展，主要包括黄烷酮、黄酮、异黄酮、黄酮醇、黄烷醇、花青素等。本论文重点研究了黄酮类化合物生物合成途径的构建和优化策略，以提高黄酮类化合物的效价和产量。然后，我们讨论了当前的挑战和未来的前景，成功的菌株开发，大规模生产黄酮类化合物在工业水平。
Flavonoids are a class of secondary metabolites found in plant and fungus. They have been widely used in food, pharmaceutical, and nutraceutical industries owing to their significant biological activities, such as antiaging, antioxidant, anti-inflammatory, and anticancer. However, the traditional approaches for the production of flavonoids including chemical synthesis and plant extraction involved hazardous materials and complicated processes and also suffered from low product titer and yield. Microbial synthesis of flavonoids from renewable biomass such as glucose and xylose has been considered as a sustainable and environmentally friendly method for large-scale production of flavonoids. Recently, construction of microbial cell factories for efficient biosynthesis of flavonoids has gained much attention. In this article, we summarize the recent advances in microbial synthesis of flavonoids including flavanones, flavones, isoflavones, flavonols, flavanols, and anthocyanins. We put emphasis on developing pathway construction and optimization strategies to biosynthesize flavonoids and to improve their titer and yield. Then, we discuss the current challenges and future perspectives on successful strain development for large-scale production of flavonoids in an industrial level.
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DOI：
10.1111/j.1432-1033.1986.tb09616.x
发表时间：
1986-05-02
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
BRITSCH, L;GRISEBACH, H
通讯作者：
GRISEBACH, H
影响因子：
8.1
作者：
Di Majo, D;Giammanco, M;Finotti, E
通讯作者：
Finotti, E
影响因子：
6.4
作者：
Cress BF;Leitz QD;Kim DC;Amore TD;Suzuki JY;Linhardt RJ;Koffas MA
通讯作者：
Koffas MA
影响因子：
4.3
作者：
Bowen-Forbes, Camille S.;Zhang, Yanjun;Nair, Muraleedharan G.
通讯作者：
Nair, Muraleedharan G.
影响因子：
8.4
作者：
Akdemir, Hulya;Silva, Adilson;Koffas, Mattheos A. G.
通讯作者：
Koffas, Mattheos A. G.