Asymmetric adhesion of rod-shaped bacteria controls microcolony morphogenesis.
Asymmetric adhesion of rod-shaped bacteria controls microcolony morphogenesis.
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DOI：
10.1038/s41467-018-03446-y
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发表时间：
2018-03-16
影响因子：
16.6
通讯作者：
Desprat N
中科院分区：
文献类型：
--
作者：
Duvernoy MC;Mora T;Ardré M;Croquette V;Bensimon D;Quilliet C;Ghigo JM;Balland M;Beloin C;Lecuyer S;Desprat N
Surface colonization underpins microbial ecology on terrestrial environments. Although factors that mediate bacteria–substrate adhesion have been extensively studied, their spatiotemporal dynamics during the establishment of microcolonies remains largely unexplored. Here, we use laser ablation and force microscopy to monitor single-cell adhesion during the course of microcolony formation. We find that adhesion forces of the rod-shaped bacteria Escherichia coli and Pseudomonas aeruginosa are polar. This asymmetry induces mechanical tension, and drives daughter cell rearrangements, which eventually determine the shape of the microcolonies. Informed by experimental data, we develop a quantitative model of microcolony morphogenesis that enables the prediction of bacterial adhesion strength from simple time-lapse measurements. Our results demonstrate how patterns of surface colonization derive from the spatial distribution of adhesive factors on the cell envelope. It is unclear how cell adhesion and elongation coordinate during formation of bacterial microcolonies. Here, Duvernoy et al. monitor microcolony formation in rod-shaped bacteria, and show that patterns of surface colonization derive from the spatial distribution of adhesive factors on the cell envelope.
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DOI：
10.1126/science.1222981
发表时间：
2012-07-13
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Berk V;Fong JC;Dempsey GT;Develioglu ON;Zhuang X;Liphardt J;Yildiz FH;Chu S
通讯作者：
Chu S
影响因子：
14.9
作者：
Chaveroche, Marie-Kim;Ghigo, Jean-Marc;d'Enfert, Christophe
通讯作者：
d'Enfert, Christophe
影响因子：
3.2
作者：
Agladze, K;Wang, X;Romeo, T
通讯作者：
Romeo, T
在杆状革兰氏阴性细菌中维持细胞形状的机制。
影响因子：
3.6
作者：
Furchtgott L;Wingreen NS;Huang KC
通讯作者：
Huang KC
影响因子：
3.7
作者：
Berne C;Ducret A;Hardy GG;Brun YV
通讯作者：
Brun YV