Metabolomics Analysis of DRG and Serum in the CCI Model of Mice.
Metabolomics Analysis of DRG and Serum in the CCI Model of Mice.
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DOI：
10.3390/brainsci13081224
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发表时间：
2023-08-21
期刊：
影响因子：
3.3
通讯作者：
Huang L
中科院分区：
文献类型：
--
作者：
Lu K;Fang B;Liu Y;Xu F;Zhou C;Wang L;Chen L;Huang L
神经病理性疼痛是一种广泛存在于普通人群中的慢性难治性疾病。它通过改变物质的代谢，导致痛苦的行为，甚至情绪变化，如焦虑和抑郁。然而，已经有有限的代谢组学研究进行有关神经性疼痛。因此，在本研究中，NP对血清和背根神经节（DRG）中代谢物的影响进行了研究，使用气相色谱-质谱（GC-MS）和液相色谱-质谱（LC-MS）检测的非靶向代谢组学方法，以揭示与NP相关的差异代谢物和受影响的代谢途径。将60只小鼠分为坐骨神经慢性压迫性损伤（CCI）组和假手术组（Sham），每组30只。CCI建模7天后，使用GC/LC-MS分析CCI和假手术组小鼠的血清和DRG的代谢产物谱。多变量分析显示CCI组和假手术组之间代谢物差异和代谢途径改变。在CCI组，我们的研究结果提供了深入了解复杂的磷脂，氨基酸和酰基肉毒碱代谢紊乱的DRG代谢。此外，在血清中观察到磷脂代谢紊乱和葡萄糖代谢受损。此外，DRG和血清中的代谢差异彼此相关。这项非靶向代谢组学研究的结果为NP对血清和DRG的代谢影响提供了视角。
Neuropathic pain (NP) is a chronic and intractable disease that is widely present in the general population. It causes painful behavior and even mood changes such as anxiety and depression by altering the metabolism of substances. However, there have been limited metabolomics studies conducted in relation to neuropathic pain. Therefore, in this study, the effects of NP on metabolites in serum and the dorsal root ganglion (DRG) were investigated using a non-targeted metabolomics approach detected by gas chromatography–mass spectrometry (GC-MS) and liquid chromatography–mass spectrometry (LC-MS) to uncover differential metabolites and affected metabolic pathways associated with NP. Sixty mice were divided into the following two groups: a chronic constriction injury (CCI) of the sciatic nerve group and a sham group (n = 30, each). After 7 days of CCI modeling, the metabolite profiles of serum and the DRG were analyzed using GC/LC-MS for both the CCI and sham groups of mice. Multivariate analysis revealed differential metabolites and altered metabolic pathways between the CCI and sham groups. In the CCI group, our findings provided insights into the complex phospholipid, amino acid and acylcarnitine metabolic perturbations of DRG metabolism. In addition, phospholipid metabolic disorders and impaired glucose metabolism were observed in the serum. Moreover, the metabolic differences in the DRG and serum were correlated with each other. The results from this untargeted metabolomics study provide a perspective on the metabolic impact of NP on serum and the DRG.
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影响因子：
3.7
作者：
Avila-Martin G;Galan-Arriero I;Gómez-Soriano J;Taylor J
通讯作者：
Taylor J
DOI：
10.1111/jon.12463
发表时间：
2017-11
期刊：
Journal of neuroimaging : official journal of the American Society of Neuroimaging
影响因子：
--
作者：
Maudsley AA;Govind V;Saigal G;Gold SG;Harris L;Sheriff S
通讯作者：
Sheriff S
DOI：
10.3390/ijerph19116698
发表时间：
2022-05-30
影响因子：
--
作者：
Cuevas-Cervera, Maria;Javier Perez-Montilla, Jose;Gonzalez-Munoz, Ana;Carmen Garcia-Rios, Maria;Navarro-Ledesma, Santiago
通讯作者：
Navarro-Ledesma, Santiago
影响因子：
4.7
作者：
Neale JH;Olszewski RT;Zuo D;Janczura KJ;Profaci CP;Lavin KM;Madore JC;Bzdega T
通讯作者：
Bzdega T
影响因子：
5.9
作者：
Maddox EK;Massoni SC;Hoffart CM;Takata Y
通讯作者：
Takata Y