Classification of fungal and bacterial lytic polysaccharide monooxygenases.
Classification of fungal and bacterial lytic polysaccharide monooxygenases.
复制标题
DOI：
10.1186/s12864-015-1601-6
复制
发表时间：
2015-05-09
期刊：
影响因子：
4.4
通讯作者：
Lange L
中科院分区：
文献类型：
--
作者：
Busk PK;Lange L
多糖单加氧酶是分解植物细胞壁和几丁质聚合物等难降解生物大分子的重要酶。这些酶最初被命名为糖苷水解酶家族61和碳水化合物结合模块家族33，但现在在CAZy数据库中被归类为辅助活性9、10和11。为了对裂解多糖单加氧酶的不同家族进行系统分析，我们利用多肽模式识别技术将AA9（1828蛋白）、AA10（2799蛋白）和AA11（769蛋白）家族中的5396个类似酶的蛋白序列划分为亚家族。结果表明，多糖单加氧酶具有两个由AA家族特有的保守肽鉴定的保守区。这些多肽用于在79个真菌和95个细菌基因组中发现裂解多糖单加氧酶。细菌基因组编码0 - 7个aa10（平均0.6）。细菌中未检出AA9和AA11。真菌基因组编码0 ~ 40个aa9序列（平均7个）和0 ~ 15个aa11序列（平均2个），其中2个真菌具有一个推定编码AA10的基因。AA9主要存在于植物细胞壁降解的asco和担子菌中，这与AA9酶的作用一致。相比之下，AA11蛋白在39个子囊菌中的36个和32个担子菌中的2个中被发现，并且它们的丰度与纤维素和半纤维素的降解无关。这些结果对不同的AA9、AA10和AA11家族的序列特征和发生进行了综述，为系统地研究多糖单加氧酶及其结构功能研究奠定了基础。本文的在线版本（doi:10.1186/s12864-015-1601-6）包含补充材料，可供授权用户使用。
Lytic polysaccharide monooxygenases are important enzymes for the decomposition of recalcitrant biological macromolecules such as plant cell wall and chitin polymers. These enzymes were originally designated glycoside hydrolase family 61 and carbohydrate-binding module family 33 but are now classified as auxiliary activities 9, 10 and 11 in the CAZy database. To obtain a systematic analysis of the divergent families of lytic polysaccharide monooxygenases we used Peptide Pattern Recognition to divide 5396 protein sequences resembling enzymes from families AA9 (1828 proteins), AA10 (2799 proteins) and AA11 (769 proteins) into subfamilies. The results showed that the lytic polysaccharide monooxygenases have two conserved regions identified by conserved peptides specific for each AA family. The peptides were used for in silico PCR discovery of the lytic polysaccharide monooxygenases in 79 fungal and 95 bacterial genomes. The bacterial genomes encoded 0 – 7 AA10s (average 0.6). No AA9 or AA11 were found in the bacteria. The fungal genomes encoded 0 – 40 AA9s (average 7) and 0 – 15 AA11s (average 2) and two of the fungi possessed a gene encoding a putative AA10. The AA9s were mainly found in plant cell wall-degrading asco- and basidiomycetes in agreement with the described role of AA9 enzymes. In contrast, the AA11 proteins were found in 36 of the 39 ascomycetes and in only two of the 32 basidiomycetes and their abundance did not correlate to the degradation of cellulose and hemicellulose. These results provides an overview of the sequence characteristics and occurrence of the divergent AA9, AA10 and AA11 families and pave the way for systematic investigations of the of lytic polysaccharide monooxygenases and for structure-function studies of these enzymes. The online version of this article (doi:10.1186/s12864-015-1601-6) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
15
作者：
Hemsworth, Glyn R.;Taylor, Edward J.;Kim, Robbert Q.;Gregory, Rebecca C.;Lewis, Sally J.;Turkenburg, Johan P.;Parkin, Alison;Davies, Gideon J.;Walton, Paul H.
通讯作者：
Walton, Paul H.
DOI：
10.1073/pnas.1323629111
发表时间：
2014-04-29
影响因子：
11.1
作者：
Agger, Jane W.;Isaksen, Trine;Westereng, Bjorge
通讯作者：
Westereng, Bjorge
影响因子：
3.4
作者：
Aspeborg H;Coutinho PM;Wang Y;Brumer H 3rd;Henrissat B
通讯作者：
Henrissat B
影响因子：
4.4
作者：
Busk, Peter Kamp;Lange, Lene
通讯作者：
Lange, Lene
影响因子：
3.7
作者：
Busk PK;Lange L
通讯作者：
Lange L