Effects of CEACAM1 in oral keratinocytes on HO-1 expression induced by Candida β-glucan particles.
Effects of CEACAM1 in oral keratinocytes on HO-1 expression induced by Candida β-glucan particles.
复制标题
CEACAM1在口服角质形成细胞中的影响对念珠菌β-葡聚糖颗粒诱导的HO-1表达。
DOI：
10.1590/1678-7757-2022-0158
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发表时间：
2022
影响因子：
2.7
通讯作者：
Aikawa, Tomonao
中科院分区：
文献类型：
--
作者：
Sakuma, Miyuki;Ohta, Kouji;Fukada, Shohei;Akagi, Misaki;Kato, Hiroki;Ishida, Yoko;Naruse, Takako;Takechi, Masaaki;Shigeishi, Hideo;Nishi, Hiromi;Aikawa, Tomonao
癌胚抗原相关细胞粘附分子1（CEACAM 1）是癌胚抗原家族的成员。虽然CEACAM 1在慢性口腔炎性上皮中有表达，但本研究旨在了解CEACAM 1在口腔角质形成细胞中是否参与了宿主对白色念珠菌的免疫应答。 我们研究了C.白色念珠菌以及念珠菌细胞壁成分β-葡聚糖颗粒（β-GPs）。进一步研究CEACAM 1对β-GP诱导的血红素加氧酶-1（HO-1）表达及其相关信号的影响。 荧光染色显示CEACAM 1在口腔角质形成细胞（RT 7）中表达，而定量逆转录（RT）-PCR显示活的和热杀死的C.白色念珠菌可增加RT 7细胞CEACAM 1 mRNA的表达。采用定量RT-PCR和Western blotting检测表明，CEACAM 1的表达也被C.白色念珠菌CEACAM 1特异性siRNA可降低C.白色念珠菌以及芽殖酵母微生物酿酒酵母（Saccharomyces cerevisiae）。此外，敲低CEACAM 1可降低β-GP诱导的早期ROS活性和Nrf 2向细胞核的转运。 口腔角质形成细胞中的CEACAM 1可能在念珠菌感染时调节HO-1对宿主免疫防御的关键作用。
Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is a member of the carcinoembryonic antigen family. Although its expression has been found in chronic oral inflammatory epithelium, this study aimed to know whether CEACAM1 in oral keratinocytes participates in host immune response against Candida albicans . We investigated CEACAM1 expression in oral keratinocytes induced by C. albicans as well as by Candida cell wall component β-glucan particles (β-GPs). Furthermore, the effects of CEACAM1 on β-GPs-induced heme oxygenase-1 (HO-1) expression and its related signals were examined. Fluorescence staining showed CEACAM1 expression in oral keratinocytes (RT7) cells, whereas quantitative reverse transcription (RT)-PCR indicated that both live and heat-killed C. albicans increased CEACAM1 mRNA expression in RT7 cells. Examinations using quantitative RT-PCR and western blotting indicated that CEACAM1 expression was also increased by β-GPs derived from C. albicans . Specific siRNA for CEACAM1 decreased HO-1 expression induced by β-GPs from C. albicans as well as the budding yeast microorganism Saccharomyces cerevisiae . Moreover, knockdown of CEACAM1 decreased β-GPs-induced ROS activity in the early phase and translocation of Nrf2 into the nucleus. CEACAM1 in oral keratinocytes may have a critical role in regulation of HO-1 for host immune defense during Candida infection.
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影响因子：
7.3
作者：
Carasi, Paula;Rodriguez, Ernesto;Freire, Teresa
通讯作者：
Freire, Teresa
影响因子：
--
作者：
Hollandsworth, Hannah M;Amirfakhri, Siamak;Bouvet, Michael
通讯作者：
Bouvet, Michael
影响因子：
4.8
作者：
Muenzner, P;Naumann, M;Gray-Owen, SD
通讯作者：
Gray-Owen, SD
影响因子：
1.9
作者：
Huynh-Torlakovic, Hong;Bjerkan, Louise;Blix, Inger J. S.
通讯作者：
Blix, Inger J. S.
影响因子：
5.8
作者：
Bryan, Holly K.;Olayanju, Adedamola;Park, B. Kevin
通讯作者：
Park, B. Kevin