Proliferating coacervate droplets as the missing link between chemistry and biology in the origins of life.
Proliferating coacervate droplets as the missing link between chemistry and biology in the origins of life.
复制标题
在生命起源中，不断扩散的凝聚液滴是化学和生物之间缺失的一环。
DOI：
10.1038/s41467-021-25530-6
复制
发表时间：
2021-09-24
影响因子：
16.6
通讯作者：
Kurihara K
中科院分区：
文献类型：
--
作者：
Matsuo M;Kurihara K
大约100年前，第一次提出了这样的假设，即益生元分子转化为聚合物，聚合物进化为增殖的分子组合，最终形成原始细胞。然而，据我们所知，还没有一个增殖的益生元系统的模型已经实现，因为聚合物的产生和自组装需要不同的条件。在这项研究中，我们确定了适合同时肽生成和自组装的条件，我们展示了如何通过使用合成的氨基酸硫酯作为益生元单体来创建基于增殖肽的液滴。由单体产生的寡肽在水中通过液-液相分离自发形成液滴。通过自催化自我繁殖定期添加单体，液滴经历了稳定的生长-分裂循环。RNA和脂质在液滴内的异质富集使得RNA能够保护液滴免于被脂质溶解。这些结果为生命起源研究提供了实验构建，并为基于肽的材料的开发开辟了方向。凝聚液滴（CD）是通过液-液相分离（LLPS）形成的原始细胞的模型，但是能够增殖的原始细胞模型仍然未开发。在这里，作者报告了一种使用合成的氨基酸硫酯作为单体的增殖肽基CD，它可以浓缩RNA和脂质，使RNA能够保护液滴免受脂质溶解。
The hypothesis that prebiotic molecules were transformed into polymers that evolved into proliferating molecular assemblages and eventually a primitive cell was first proposed about 100 years ago. To the best of our knowledge, however, no model of a proliferating prebiotic system has yet been realised because different conditions are required for polymer generation and self-assembly. In this study, we identify conditions suitable for concurrent peptide generation and self-assembly, and we show how a proliferating peptide-based droplet could be created by using synthesised amino acid thioesters as prebiotic monomers. Oligopeptides generated from the monomers spontaneously formed droplets through liquid–liquid phase separation in water. The droplets underwent a steady growth–division cycle by periodic addition of monomers through autocatalytic self-reproduction. Heterogeneous enrichment of RNA and lipids within droplets enabled RNA to protect the droplet from dissolution by lipids. These results provide experimental constructs for origins-of-life research and open up directions in the development of peptide-based materials. Coacervate droplets (CDs) are a model for protocells formed by liquid-liquid phase separation (LLPS), but protocell models able to proliferate remain undeveloped. Here, the authors report a proliferating peptide-based CD using synthesised amino acid thioesters as monomers, which could concentrate RNA and lipids, enabling RNA to protect the droplet from dissolution by lipids.
登录
查看更多内容
影响因子：
15
作者：
Forbes CR;Sinha SK;Ganguly HK;Bai S;Yap GP;Patel S;Zondlo NJ
通讯作者：
Zondlo NJ
影响因子：
21.8
作者：
Kurihara, Kensuke;Tamura, Mieko;Sugawara, Tadashi
通讯作者：
Sugawara, Tadashi
影响因子：
64.8
作者：
FOX, SW
通讯作者：
FOX, SW
DOI：
10.1073/pnas.63.4.1335
发表时间：
1969-01-01
影响因子：
11.1
作者：
GEVERS, W;KLEINKAUF, H;LIPMANN, F
通讯作者：
LIPMANN, F
影响因子：
4.6
作者：
Camprubi, Eloi;Jordan, Sean F.;Lane, Nick
通讯作者：
Lane, Nick