Investigation of possible molecular mechanisms underlying the regulation of adhesion in Vibrio alginolyticus with comparative transcriptome analysis.
Investigation of possible molecular mechanisms underlying the regulation of adhesion in Vibrio alginolyticus with comparative transcriptome analysis.
复制标题
通过比较转录组分析研究溶藻弧菌粘附调节的可能分子机制
DOI：
10.1007/s10482-015-0411-9
复制
发表时间：
2015-05
影响因子：
2.6
通讯作者：
Yan, Qingpi
中科院分区：
文献类型：
--
作者：
Kong, Wendi;Huang, Lixing;Su, Yongquan;Qin, Yingxue;Ma, Ying;Xu, Xiaojin;Lin, Mao;Zheng, Jiang;Yan, Qingpi
对鱼黏液的粘附能力受环境条件的影响，被认为是溶藻弧菌的关键毒力因素，但其分子机制尚不清楚。本研究采用Cu2+ (50 mg/L)、Pb2+ (100 mg/L)、Hg2+ (50 mg/L)和低pH （pH 5）胁迫条件对藻毒弧菌进行处理。我们发现这些胁迫处理能够降低褐藻溶解弧菌的黏附，并且根据高通量测序结果，多个基因的表达水平发生了显著变化。随机选择的基因通过QPCR证实表达，增强了测序数据的可靠性。本体分配和KEGG通路分析表明，应激处理影响可能与粘附有关的通路。本研究结果鉴定出了可能在溶藻弧菌粘附过程中起关键作用的基因，为进一步分析这些基因在粘附过程中的功能奠定了基础。由于这些基因对环境因素敏感，这可能解释了为什么粘附过程会受到环境因素的影响。本文的在线版本（doi:10.1007/s10482-015-0411-9）包含补充材料，可供授权用户使用。
Adhesion capability to fish mucus, which can be affected by environmental conditions, is considered to be a key virulence factor of Vibrio alginolyticus although the molecular mechanism is still unclear. In the present study, V. alginolyticus was treated with stress conditions including Cu2+ (50 mg/L), Pb2+ (100 mg/L), Hg2+ (50 mg/L) and low pH (pH 5). We found these stress treatments were capable of reducing the adhesion of V. alginolyticus, while the expression levels of multiple genes were significantly changed according to the results of high throughput sequencing. The expression of randomly selected genes was confirmed by QPCR, which reinforced the reliability of the sequencing data. Ontology assignments and KEGG pathway analysis indicated that stress treatments affect pathways that may be related to adhesion. Our results identified genes which might play a key role in the adhesion process of V. alginolyticus, which could lay a foundation for further functional analysis of these genes in the process of adhesion. As these genes were sensitive to environmental factors, this may explain why the adhesion process can be influenced by environmental factors. The online version of this article (doi:10.1007/s10482-015-0411-9) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
3
作者：
Reddy JS;Kumar R;Watt JM;Lawrence ML;Burgess SC;Nanduri B
通讯作者：
Nanduri B
影响因子：
3.4
作者：
Mello, Bernardo A.;Tu, Yuhai
通讯作者：
Tu, Yuhai
影响因子：
4.4
作者：
Bordas, MA;Balebona, MC;Morinigo, MA
通讯作者：
Morinigo, MA
影响因子：
3.2
作者：
BELAS, MR;COLWELL, RR
通讯作者：
COLWELL, RR
DOI：
10.1007/bf00407699
发表时间：
1971-01-01
期刊：
ARCHIV FUR MIKROBIOLOGIE
影响因子：
--
作者：
MEADOWS, PS
通讯作者：
MEADOWS, PS