Bacillus cereus (EG-Q3) in the Gut of Ectropis grisescens Contributes to Host Response to Starvation Conditions.
Bacillus cereus (EG-Q3) in the Gut of Ectropis grisescens Contributes to Host Response to Starvation Conditions.
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灰褐苋肠道中的蜡状芽孢杆菌 (EG-Q3) 有助于宿主对饥饿条件的反应
DOI：
10.3389/fmicb.2022.785415
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发表时间：
2022
影响因子：
5.2
通讯作者：
Long, Yanhua
中科院分区：
文献类型：
--
作者：
Li, Xiayu;Zhang, Yong;Zhou, Linlin;Gao, Tian;Zhao, Yu;Liu, Song;Su, Qingqing;Wei, Chaoling;Yang, Yunqiu;Long, Yanhua
昆虫肠道细菌在昆虫的营养、维持和生态适应中起着重要作用。灰飞虱是中国茶园最重要的食叶性害虫。为了探索灰霉在饥饿胁迫下的适应性是否与其肠道细菌有关，我们采用非培养的方法比较了饥饿处理下其肠道细菌的组成和多样性。结果表明，饥饿后24 h内，核心肠道细菌组成和多样性没有明显变化。而非核心肠道细菌在饥饿条件下显著增加。蜡样芽孢杆菌EG-Q3菌株在碳源选择的条件下，在体外和体内均能降解稻瘟病菌的脂肪体。此外，在饥饿4h后，添加蜡样芽孢杆菌EG-Q3菌株的灰指肠杆菌的降脂率(6.76±1.281%)显著高于对照组(3.96±0.801%，t=4.15，df=8，p<；0.01)，这些结果表明非核心肠道细菌EG-Q3菌株有助于宿主对饥饿的适应。总之，这项研究提供了证据，表明在饥饿条件下，灰色E.grisescens可能受益于非核心肠道细菌。
The gut bacteria of insects play an important role in their nutrition, maintenance, and ecological adaption. Ectropis grisescens is the most important leaf-feeding pest in tea gardens in China. In order to explore whether E. grisescens adaptation under starvation stress is related to its gut bacteria, we used a culture-independent method to compare the composition and diversity of their gut bacteria under starvation treatment. The results revealed no significant changes in core gut bacteria composition and diversity within 24 h of starvation. However, non-core gut bacterial Bacillus increased significantly under starvation conditions. B. cereus strain EG-Q3 isolated from the gut of E. grisescens in carbon source-selected medium showed the ability to degrade fat bodies from E. grisescens in vitro and in vivo. Moreover, the fat-lowering ratio of E. grisescens fed with B. cereus strain EG-Q3 (6.76 ± 1.281%) was significantly higher than that of the control group (3.96 ± 0.801%, t = 4.15, df = 8, p < 0.01) after starvation for 4 h. These findings suggest that non-core gut bacterial B. cereus strain EG-Q3 contributes to host adaptation to starvation. Together, this research provides evidence that E. grisescens may benefit from non-core gut bacteria under starvation conditions.
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影响因子：
4.6
作者：
Ahmad M;Chaudhary SU;Afzal AJ;Tariq M
通讯作者：
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影响因子：
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通讯作者：
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