Overexpression of AtTTP affects ARF17 expression and leads to male sterility in Arabidopsis.
Overexpression of AtTTP affects ARF17 expression and leads to male sterility in Arabidopsis.
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DOI：
10.1371/journal.pone.0117317
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Yang ZN
中科院分区：
文献类型：
--
作者：
Shi ZH;Zhang C;Xu XF;Zhu J;Zhou Q;Ma LJ;Niu J;Yang ZN
胼胝质的合成对花粉壁型的形成至关重要。CalS5被认为是胼胝质壁的主要合成酶。在拟南芥花药中，ARF17调控CalS5的表达，是miR160的靶点。表达mir160抗性ARF17 （35S:5mARF17系）且ARF17 mRNA水平升高的植株表现为雄性不育。在这里，我们报道了一个锌指家族基因AtTTP，它参与拟南芥miR160的成熟和胼胝质的合成。AtTTP在花药的小孢子细胞、四分体和绒毡层细胞中表达。AtTTP过表达系（AtTTP- oe系）表现出雄性生育能力降低。在attp - oe细胞系中，CalS5表达量显著降低，四分体胼胝质壁变薄。Northern blotting杂交和定量RT-PCR分析显示，attp - oe细胞系miR160表达降低，ARF17表达升高。基于这些结果，我们认为AtTTP与miR160相关，从而调控胼胝质合成和花粉壁形成所需的ARF17表达。
Callose synthesis is critical for the formation of the pollen wall pattern. CalS5 is thought to be the major synthethase for the callose wall. In the Arabidopsis anther, ARF17 regulates the expression of CalS5 and is the target of miR160. Plants expressing miR160-resistant ARF17 (35S:5mARF17 lines) with increased ARF17 mRNA levels display male sterility. Here we report a zinc finger family gene, AtTTP, which is involved in miR160 maturation and callose synthesis in Arabidopsis. AtTTP is expressed in microsporocytes, tetrads and tapetal cells in the anther. Over-expression lines of AtTTP (AtTTP-OE line) exhibited reduced male fertility. CalS5 expression was tremendously reduced and the tetrad callose wall became much thinner in the AtTTP-OE line. Northern blotting hybridization and quantitative RT-PCR analysis revealed that miR160 was decreased, while the expression of ARF17 was increased in the AtTTP-OE line. Based on these results, we propose that AtTTP associates with miR160 in order to regulate the ARF17 expression needed for callose synthesis and pollen wall formation.
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