Callose (beta-1,3 glucan) is essential for Arabidopsis pollen wall patterning, but not tube growth.
Callose (beta-1,3 glucan) is essential for Arabidopsis pollen wall patterning, but not tube growth.
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DOI：
10.1186/1471-2229-5-22
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发表时间：
2005-10-07
影响因子：
5.3
通讯作者：
Preuss D
中科院分区：
文献类型：
--
作者：
Nishikawa S;Zinkl GM;Swanson RJ;Maruyama D;Preuss D
胼胝质（β-1，3-葡聚糖）分离发育中的花粉粒，防止它们下面的壁（外壁）融合。将精子运送到雌配子的花粉管也含有胼胝质，既在它们的壁中，也在分割生长管的塞子中。CalS 5是几种拟南芥β-1，3葡聚糖糖苷酶之一，其突变先前显示破坏发育中的小孢子周围的胼胝质形成，导致外壁图案畸变，发育中的小孢子退化和花粉不育。在这里，我们描述了三个额外的cals 5等位基因，同样改变外壁模式，但不是产生可育花粉。此外，这些等位基因之一（cals 5 -3）导致形成的花粉管缺乏胼胝质壁和塞子。在自花授粉植物中，这些管导致成功受精，但它们在与野生型竞争时处于轻微劣势。与以前的报道相反，这些结果表明，花粉发育，生活力或生育力不需要结构化外壁层。此外，尽管存在胼胝质丰富的壁和胼胝质塞在花粉管中，这里提出的结果表明，胼胝质是不需要的花粉管功能。
Callose (β-1,3 glucan) separates developing pollen grains, preventing their underlying walls (exine) from fusing. The pollen tubes that transport sperm to female gametes also contain callose, both in their walls as well as in the plugs that segment growing tubes. Mutations in CalS5, one of several Arabidopsis β-1,3 glucan synthases, were previously shown to disrupt callose formation around developing microspores, causing aberrations in exine patterning, degeneration of developing microspores, and pollen sterility. Here, we describe three additional cals5 alleles that similarly alter exine patterns, but instead produce fertile pollen. Moreover, one of these alleles (cals5-3) resulted in the formation of pollen tubes that lacked callose walls and plugs. In self-pollinated plants, these tubes led to successful fertilization, but they were at a slight disadvantage when competing with wild type. Contrary to a previous report, these results demonstrate that a structured exine layer is not required for pollen development, viability or fertility. In addition, despite the presence of callose-enriched walls and callose plugs in pollen tubes, the results presented here indicate that callose is not required for pollen tube functions.
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