Effects of CYP-induced cystitis on PACAP/VIP and receptor expression in micturition pathways and bladder function in mice with overexpression of NGF in urothelium.
Effects of CYP-induced cystitis on PACAP/VIP and receptor expression in micturition pathways and bladder function in mice with overexpression of NGF in urothelium.
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DOI：
10.1007/s12031-012-9834-1
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发表时间：
2012-11
影响因子：
3.1
通讯作者：
Vizzard, Margaret A.
中科院分区：
文献类型：
--
作者：
Girard, Beatrice M.;Tompkins, John D.;Parsons, Rodney L.;May, Victor;Vizzard, Margaret A.
We have previously demonstrated nerve growth factor (NGF) regulation of pituitary adenylate cyclase-activating polypeptide (PACAP)/receptors in bladder reflex pathways using a transgenic mouse model of chronic NGF overexpression in the bladder using the urothelial-specific uroplakin II promoter. We have now explored the contribution of target-derived NGF in combination with cyclophosphamide (CYP)-induced cystitis to determine whether additional changes in neuropeptides/receptors are observed in micturition reflex pathways due to the presence of additional inflammatory mediators in the urinary bladder. Quantitative PCR was used to determine PACAP/vasoactive intestinal polypeptide (VIP), substance P, galanin, and receptor transcript expression in the urinary bladder (urothelium, detrusor) in mice with overexpression of NGF in the urothelium (NGF-OE) and wild-type (WT) mice with CYP-induced cystitis (4 h, 48 h, and chronic). With CYP-induced cystitis (4 h), WT and NGF-OE mice exhibited similar changes in galanin transcript expression in the urothelium (30-fold increase) and detrusor (threefold increase). In contrast, PACAP, VIP, and substance P transcripts exhibited differential changes in WT and NGF-OE with CYP-induced cystitis. PAC1, VPAC1, and VPAC2 transcript expression also exhibited differential responses in NGF-OE mice that were tissue (urothelium vs. detrusor) and CYP-induced cystitis duration-dependent. Using conscious cystometry, NGF-OE mice treated with CYP exhibited significant (p≤0.01) increases in voiding frequency above that observed in control NGF-OE mice. In addition, no changes in the electrical properties of the major pelvic ganglia neurons of NGF-OE mice were detected using intracellular recording, suggesting that the urinary bladder phenotype in NGF-OE mice is not influenced by changes in the efferent limb of the micturition reflex. These studies are consistent with target-derived NGF and other inflammatory mediators affecting neurochemical plasticity and the reflex function of micturition pathways.
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影响因子：
6.6
作者：
Driscoll, A;Teichman, JMH
通讯作者：
Teichman, JMH
影响因子：
6.6
作者：
Evans, R. J.;Moldwin, R. M.;Scholfield, D.
通讯作者：
Scholfield, D.
影响因子：
4.7
作者：
Girard, Beatrice A.;Lelievre, Vincent;May, Victor
通讯作者：
May, Victor
DOI：
10.1152/ajprenal.90305.2008
发表时间：
2008-09-01
影响因子：
4.2
作者：
Cheppudira, Bopaiah P.;Girard, Beatrice M.;Vizzard, Margaret A.
通讯作者：
Vizzard, Margaret A.
DOI：
10.1007/bf01605152
发表时间：
1990-03-01
期刊：
VIRCHOWS ARCHIV A-PATHOLOGICAL ANATOMY AND HISTOPATHOLOGY
影响因子：
--
作者：
CHRISTMAS, TJ;RODE, J;TURNERWARWICK, RT
通讯作者：
TURNERWARWICK, RT