Specific Acetylation Patterns of H2A.Z Form Transient Interactions with the BPTF Bromodomain.
Specific Acetylation Patterns of H2A.Z Form Transient Interactions with the BPTF Bromodomain.
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H2A.Z 的特定乙酰化模式与 BPTF 布罗莫结构域形成瞬时相互作用。
DOI：
10.1021/acs.biochem.7b00648
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发表时间：
2017
期刊：
影响因子：
2.9
通讯作者：
Pomerantz,WilliamCK
中科院分区：
文献类型：
--
作者：
Perell,GabriellaT;Mishra,NeerajK;Sudhamalla,Babu;Ycas,PeterD;Islam,Kabirul;Pomerantz,WilliamCK
Post-translational lysine acetylation of histone tails affects both chromatin accessibility and recruitment of multifunctional bromodomain-containing proteins for modulating transcription. The bromodomain- and PHD finger-containing transcription factor (BPTF) regulates transcription but has also been implicated in high gene expression levels in a variety of cancers. In this report, the histone variant H2A.Z, which replaces H2A in chromatin, is evaluated for its affinity for BPTF with a specific recognition pattern of acetylated lysine residues of the N-terminal tail region. Although BPTF immunoprecipitates H2A.Z-containing nucleosomes, a direct interaction with its bromodomain has not been reported. Using protein-observed fluorine nuclear magnetic resonance (PrOF NMR) spectroscopy, we identified a diacetylation of H2A.Z on lysine residues 4 and 11, with the highest affinity for BPTF with aKdof 780 μM. A combination of subsequent1H NMR Carr–Purcell–Meiboom–Gill experiments and photo-cross-linking further confirmed the specificity of the diacetylation pattern at lysines 4 and 11. Because of an adjacent PHD domain, this transient interaction may contribute to a higher-affinity bivalent interaction. Further evaluation of specificity toward a set of bromodomains, including two BET bromodomains (Brd4 and BrdT) and twoPlasmodium falciparumbromodomains, resulted in one midmicromolar affinity binder,PfGCN5 (Kd= 650 μM). With these biochemical experiments, we have identified a direct interaction of histone H2A.Z with bromodomains with a specific acetylation pattern that further supports the role of H2A.Z in epigenetic regulation.
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影响因子：
4
作者：
Urick AK;Calle LP;Espinosa JF;Hu H;Pomerantz WC
通讯作者：
Pomerantz WC
影响因子：
64.5
作者：
Ruthenburg AJ;Li H;Milne TA;Dewell S;McGinty RK;Yuen M;Ueberheide B;Dou Y;Muir TW;Patel DJ;Allis CD
通讯作者：
Allis CD
H2A.Z 在胚胎干细胞自我更新和分化过程中促进活性复合物和抑制复合物接触染色质。
影响因子：
23.9
作者：
Hu G;Cui K;Northrup D;Liu C;Wang C;Tang Q;Ge K;Levens D;Crane-Robinson C;Zhao K
通讯作者：
Zhao K
影响因子：
4.5
作者：
Hardy S;Jacques PE;Gévry N;Forest A;Fortin ME;Laflamme L;Gaudreau L;Robert F
通讯作者：
Robert F
影响因子：
12.3
作者：
Ku M;Jaffe JD;Koche RP;Rheinbay E;Endoh M;Koseki H;Carr SA;Bernstein BE
通讯作者：
Bernstein BE