Chronic exposure to rapamycin and episodic serum starvation modulate ageing of human fibroblasts in vitro
Chronic exposure to rapamycin and episodic serum starvation modulate ageing of human fibroblasts in vitro
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长期暴露于雷帕霉素和间歇性血清饥饿可调节体外人成纤维细胞的衰老
DOI：
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
S. Rattan
中科院分区：
文献类型：
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作者：
Lakshman Sodagam;A. Lewińska;M. Wnuk;S. Rattan
轻度应激诱导的应激反应（SR）途径的激活，如自噬、热休克反应、氧化SR、DNA损伤反应和炎症反应，可能对健康有益。利用体外细胞衰老和复制性衰老模型系统，研究了雷帕霉素和血清饥饿两种条件对衰老的调节作用。慢性暴露于0.1、1和10 nM的雷帕霉素对连续传代的正常人皮肤成纤维细胞的存活、生长、形态、端粒长度、DNA甲基化水平、DNA中8-o - dg水平、RNA中n6 -甲基腺苷水平和乙醇胁迫耐受性均有正向调节。此外，人类皮肤成纤维细胞的间歇性（每周一次）血清饥饿使其复制寿命延长了约22%，即使在传代后期培养中也能保持早期传代的年轻形态。虽然本研究的结果可能被认为是初步的，但可以推断，间歇性和间歇性诱导SR，而不是慢性上调SR，更有效和适用于健康老龄化和长寿的激效实践。
Mild stress-induced activation of stress response (SR) pathways, such as autophagy, heat shock response, oxidative SR, DNA damage response, and inflammatory response, can be potentially health beneficial. Using the model system of cellular ageing and replicative senescence in vitro, we have studied the ageing modulatory effects of the two conditions, rapamycin and serum starvation. Chronic exposure to 0.1, 1 and 10 nM rapamycin positively modulated the survival, growth, morphology, telomere length, DNA methylation levels, 8-oxo-dG level in DNA, N6-methyl-adenosine level in RNA, and ethanol stress tolerance of serially passaged normal human skin fibroblasts. Furthermore, episodic (once a week) serum starvation of human skin fibroblasts extended their replicative lifespan by about 22%, along with the maintenance of early passage youthful morphology even in late passage cultures. Although the results of this study may be considered preliminary, it can be inferred that intermittent and episodic induction of SR, rather than chronic up-regulation of SR, is more effective and applicable in the practice of hormesis for healthy ageing and longevity.
DOI：
10.1073/pnas.92.20.9363
发表时间：
1995-09-26
影响因子：
11.1
作者：
DIMRI, GP;LEE, XH;CAMPISI, J
通讯作者：
CAMPISI, J
DOI：
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发表时间：
2013-09
期刊：
Journal of biochemical and pharmacological research
影响因子：
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作者：
S. Ryter;A. Choi
通讯作者：
S. Ryter;A. Choi
影响因子：
56.9
作者：
Iyer, VR;Eisen, MB;Brown, PO
通讯作者：
Brown, PO