Plasma and follicular fluid osteopontin levels during ovarian cycle and their correlation with follicular fluid vascular endothelial growth factor levels.
Plasma and follicular fluid osteopontin levels during ovarian cycle and their correlation with follicular fluid vascular endothelial growth factor levels.
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DOI：
10.1038/s41598-020-79453-1
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发表时间：
2021-01-11
影响因子：
4.6
通讯作者：
Takeshita T
中科院分区：
文献类型：
--
作者：
Kuwabara Y;Ono S;Katayama A;Kurihara S;Oishi Y;Takeshita T
骨桥蛋白（OPN）是一种多功能分泌糖蛋白。我们评估了卵巢周期期间血液和卵泡液 (FF) 中的 OPN 浓度及其与血管内皮生长因子 (VEGF) 产生的关系，血管内皮生长因子 (VEGF) 参与卵巢过度刺激综合征 (OHSS) 的病理生理学。纳入了 22 名接受体外受精（克罗米芬柠檬酸盐最小刺激方案）的女性。 (a) 撤退性出血第三天、(b) 卵母细胞取出前 2 天、(c) 卵母细胞取出当天收集样本。 FF是在卵母细胞取出过程中收集的。通过酶联免疫吸附测定法测量每个样本中的 OPN 浓度和 FF 中的 VEGF 浓度。血浆OPN浓度（以ng/mL为单位）：（a）416±37.2，（b）378±35.8，（c）390±40.0，各组之间没有显着差异。 FF 中的 OPN 浓度为 106±13.4 ng/mL。 FF 和血浆样本中的 OPN 浓度之间存在正相关关系。血浆 OPN 和 FF VEGF 浓度之间也发现呈正相关，与采血周期无关。建议血浆 OPN 浓度反映卵母细胞取出时的 FF VEGF 水平，并且可能是预测 OHSS 风险的新临床标志物。
Osteopontin (OPN) is a multifunctional secreted glycoprotein. We evaluated OPN concentrations in blood and follicular fluid (FF) during the ovarian cycle and their relationship with the production of vascular endothelial growth factor (VEGF), which is involved in the pathophysiology of ovarian hyperstimulation syndrome (OHSS). Twenty-two women undergoing in vitro fertilization (minimal stimulation protocol with clomiphene citrate) were enrolled. Samples were collected (a) on the third day of withdrawal bleeding, (b) 2 days before oocyte retrieval, and (c) on the day of oocyte retrieval. FF was collected during oocyte retrieval. The OPN concentration in each specimen and the VEGF concentration in FF was measured by enzyme-linked immunosorbent assays. Plasma OPN concentrations were (in ng/mL): (a) 416 ± 37.2, (b) 378 ± 35.8, and (c) 390 ± 40.0, with no significant differences between the groups. The OPN concentration in FF was 106 ± 13.4 ng/mL. A positive correlation was found between OPN concentrations in FF and plasma samples. A positive correlation was also found between plasma OPN and FF VEGF concentrations, irrespective of the blood-sampling period. Plasma OPN concentration is suggested to reflect the FF VEGF level at oocyte retrieval and maybe a novel clinical marker for predicting the risk for OHSS.
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DOI：
10.1111/j.1749-6632.1995.tb44624.x
发表时间：
1995-01-01
期刊：
OSTEOPONTIN: ROLE IN CELL SIGNALLING AND ADHESION
影响因子：
--
作者：
GIACHELLI, CM;LIAW, L;ALMEIDA, M
通讯作者：
ALMEIDA, M
影响因子：
4.8
作者：
Shao, Zhifei;Morser, John;Leung, Lawrence L. K.
通讯作者：
Leung, Lawrence L. K.
影响因子：
4.1
作者：
FRANZEN, A;HEINEGARD, D
通讯作者：
HEINEGARD, D
影响因子：
3.3
作者：
BROWN, LF;BERSE, B;SENGER, DR
通讯作者：
SENGER, DR
影响因子：
6.7
作者：
Baskind, N. Ellissa;Orsi, Nicolas M.;Sharma, Vinay
通讯作者：
Sharma, Vinay