Transcription-dependent localization of U1 and U2 small nuclear ribonucleoproteins at major sites of gene activity in polytene chromosomes.
Transcription-dependent localization of U1 and U2 small nuclear ribonucleoproteins at major sites of gene activity in polytene chromosomes.
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U1 和 U2 小核核糖核蛋白在多线染色体基因活性主要位点的转录依赖性定位。
DOI：
10.1016/0022-2836(84)90263-8
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发表时间：
1984
影响因子：
5.6
通讯作者：
Pederson,T
中科院分区：
文献类型：
--
作者：
Sass,H;Pederson,T
The location and dynamics of small nuclear ribonucleoproteins (snRNPs) were studied in salivary gland polytene chromosomes ofChironomus tentansby immunofluorescence with specific snRNP antibodies. Monoclonal antibody against the snRNP Sm antigens reacted at all sites of transcription (puffs and Balbiani rings). The amount of snRNP immunofluorescence was strictly dependent on transcription, increasing in parallel with gene activation and decreasing upon repression. Identical patterns of localization and transcriptional dependence were observed with antibodies specific for U1 or U2 snRNPs. These latter results show that the involvement of U1 and U2 snRNPs in transcription-related processes involves a high proportion, rather than small subsets, of active gene loci. In addition, the colocalization of U1 and U2 snRNPs at loci known to contain only one messenger RNA transcription unit (e.g. Balbiani ring 2) raises the possibility that both of these snRNPs interact with the same transcript. Finally, the lack of immunofluorescence at repressed loci indicates that snRNPs are not structural components of the chromatin (DNP) fiber, and also shows that unused snRNPs are not stored in chromatin. These latter points, and the growing evidence for the involvement of U1 snRNP in splicing, suggest that nascent pre-mRNA is the major chromosomal binding site for snRNPs.
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DOI：
10.1002/ar.1091340308
发表时间：
1959
期刊：
The Anatomical Record
影响因子：
--
作者：
H. A. Kent
通讯作者：
H. A. Kent
用己烯雌酚治疗的新生小鼠卵巢中出现多卵泡。
DOI：
--
发表时间：
1985
期刊：
影响因子：
--
作者：
T. Iguchi
通讯作者：
T. Iguchi
DOI：
10.1093/jnci/39.1.75
发表时间：
1967
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
T. Kimura;S. Nandi
通讯作者：
S. Nandi
诱导小鼠持续阴道角化的性质。
DOI：
--
发表时间：
1967
期刊：
The Journal of experimental zoology
影响因子：
--
作者：
T. Kimura;S. Basu;S. Nandi
通讯作者：
S. Nandi
与产前接触己烯雌酚相关的对雌性小鼠生殖道的长期影响。
影响因子：
11.2
作者：
J. Mclachlan;R. Newbold;B. Bullock
通讯作者：
B. Bullock