Optical tweezers across scales in cell biology.

Optical tweezers across scales in cell biology.
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细胞生物学范围内的光学镊子。

DOI:
10.1016/j.tcb.2022.05.001
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发表时间:
2022-11
影响因子:
19
通讯作者:
Scott, Ethan K.
Scott, Ethan K.
中科院分区:
生物学1区
文献类型:
--
作者:
Favre-Bulle, Itia A.;Scott, Ethan K.

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光学镊子提供了一种非侵入性方法,用于向目标物体提供微小的物理力。在活细胞或体外制剂中控制这些力允许测量和操纵与细胞的形式和功能相关的许多过程。因此,光学镊子对我们理解蛋白质和核酸的结构、细胞内微观结构之间发生的相互作用、有丝分裂等复杂过程的编排以及细胞相互作用的方式做出了重要贡献。在这篇综述中,我们重点介绍了使用光镊在细胞生物学领域的最新贡献,并提供了使这些研究成为可能的物理、方法和设备的基本描述。
Optical tweezers provide a noninvasive approach for delivering minute physical forces to targeted objects. Controlling such forces in living cells or in vitro preparations allows for the measurement and manipulation of numerous processes relevant to the form and function of cells. As such, optical tweezers have made important contributions to our understanding of the structures of proteins and nucleic acids, the interactions that occur between microscopic structures within cells, the choreography of complex processes such as mitosis, and the ways in which cells interact with each other. In this review, we highlight recent contributions made to the field of cell biology using optical tweezers, and provide basic descriptions of the physics, the methods, and the equipment that made these studies possible.
DOI: 10.1038/srep42960
发表时间: 2017-02-21
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影响因子: 4.6
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