Serum under-O-glycosylated IgA1 level is not correlated with glomerular IgA deposition based upon heterogeneity in the composition of immune complexes in IgA nephropathy.
Serum under-O-glycosylated IgA1 level is not correlated with glomerular IgA deposition based upon heterogeneity in the composition of immune complexes in IgA nephropathy.
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DOI：
10.1186/1471-2369-15-89
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发表时间：
2014-06-13
期刊：
影响因子：
2.3
通讯作者：
Tomino Y
中科院分区：
文献类型：
--
作者：
Satake K;Shimizu Y;Sasaki Y;Yanagawa H;Suzuki H;Suzuki Y;Horikoshi S;Honda S;Shibuya K;Shibuya A;Tomino Y
尽管伊加肾病（IgAN）患者血清低O-糖基化IgA 1可能比高O-糖基化IgA 1更优先存款于肾小球，但肾小球伊加沉积水平与血清IgA 1 O-聚糖谱之间的关系尚不清楚。采用酶联免疫吸附试验（ELISA）检测32例IgAN患者血清中总的O-糖基化IgA 1水平。采用小鼠Fcα/μ受体（mFcα/μR）转染子和流式细胞术（pIgA 1 trap），通过原始方法选择性测定血清低O-糖基化多聚IgA 1（pIgA 1）。通过对活检标本的免疫荧光进行图像分析，定量整个肾小球中伊加沉积的面积百分比（面积-IgA）。评估了面积-IgA与HAA-ELISA或pIgA 1陷阱数据之间的相关性。应用数据挖掘方法分析HAA-ELISA和pIgA 1 trap检测结果与临床指标的关系。当测量时，IgAN患者中O-糖基化不足的IgA 1水平显著高于健康对照（p < 0.05），而O-糖基化不足的pIgA 1没有显著差异。在IgAN患者（r2 = 0.005）中，HAA-ELISA和pIgA 1 trap的数据（r2 = 0.09）之间既没有观察到相关性，也没有观察到面积-IgA与HAA-ELISA或pIgA 1 trap的数据之间的线性相关性（分别为r2 = 0.005，0.03）。临床参数与HAA-ELISA和pIgA 1陷阱数据的等值线图显示，每个临床参数得分高的患者集中在特定区域，表明具有特异性IgA 1 O-聚糖谱的患者具有相似的临床参数。决策树分析表明，肾小球免疫复合物主要由IgA 1-IgG和补体、pIgA 1和补体、单体IgA 1-伊加或聚集的单体IgA 1组成。基于IgAN患者肾小球免疫复合物组成的异质性，血清低-O-糖基化IgA 1水平与肾小球伊加沉积无关。
Although serum under-O-glycosylated IgA1 in IgA nephropathy (IgAN) patients may deposit more preferentially in glomeruli than heavily-O-glycosylated IgA1, the relationship between the glomerular IgA deposition level and the O-glycan profiles of serum IgA1 remains obscure. Serum total under-O-glycosylated IgA1 levels were quantified in 32 IgAN patients by an enzyme-linked immunosorbent assay (ELISA) with Helix aspersa (HAA) lectin. Serum under-O-glycosylated polymeric IgA1 (pIgA1) was selectively measured by an original method using mouse Fcα/μ receptor (mFcα/μR) transfectant and flow cytometry (pIgA1 trap). The percentage area of IgA deposition in the whole glomeruli (Area-IgA) was quantified by image analysis on the immunofluorescence of biopsy specimens. Correlations were assessed between the Area-IgA and data from HAA-ELISA or pIgA1 trap. The relationships between clinical parameters and data from HAA-ELISA or pIgA1 trap were analyzed by data mining approach. While the under-O-glycosylated IgA1 levels in IgAN patients were significantly higher than those in healthy controls when measured (p < 0.05), there was no significant difference in under-O-glycosylated pIgA1. There was neither a correlation observed between the data from HAA-ELISA and pIgA1 trap (r2 = 0.09) in the IgAN patients (r2 = 0.005) nor was there a linear correlation between Area-IgA and data from HAA-ELISA or the pIgA1 trap (r2 = 0.005, 0.03, respectively). Contour plots of clinical parameters versus data from HAA-ELISA and the pIgA1 trap revealed that patients with a high score in each clinical parameter concentrated in specific areas, showing that patients with specific O-glycan profiles of IgA1 have similar clinical parameters. A decision tree analysis suggested that dominant immune complexes in glomeruli were consisted of: 1) IgA1-IgG and complements, 2) pIgA1 and complements, and 3) monomeric IgA1-IgA or aggregated monomeric IgA1. Serum under-O-glycosylated IgA1 levels are not correlated with glomerular IgA deposition based upon heterogeneity in the composition of glomerular immune complexes in IgAN patients.
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影响因子：
2.7
作者：
Maeda, A;Gohda, T;Tomino, Y
通讯作者：
Tomino, Y
影响因子：
5.4
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Ghumra, Ashfaq;Shi, Jianguo;Mcintosh, Richard S.;Rasmussen, Ingunn B.;Braathen, Ranveig;Johansen, Finn-Eirik;Sandlie, Inger;Mongini, Patricia K.;Areschoug, Thomas;Lindahl, Gunnar;Lewis, Melanie J.;Woof, Jenny M.;Pleass, Richard J.
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