Renal proximal tubular epithelial cells: review of isolation, characterization, and culturing techniques
Renal proximal tubular epithelial cells: review of isolation, characterization, and culturing techniques
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肾近端肾小管上皮细胞：分离、表征和培养技术综述
DOI：
10.1007/s11033-020-05977-4
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发表时间：
2020
影响因子：
2.8
通讯作者：
S. Bevc
中科院分区：
文献类型：
--
作者：
Matic Mihevc;T. Petreski;U. Maver;S. Bevc
肾脏是一个复杂的器官，主要由肾小球、肾小管、系膜和内皮细胞以及足细胞组成。肾细胞在妊娠34周时终末分化的事实是再生和治疗急性肾损伤或慢性肾病的主要障碍。此外，慢性肾脏疾病患者的数量不断增加，医学界应致力于改善现有的肾脏替代疗法并开发新的方法。另一方面，随着多种药物的增加，应该考虑开发新的药物安全性测试方法。解决这些问题的一种可能方法是分离和培养肾细胞。目前描述了几种分离所需细胞的方案，其中最分离的是近端肾小管上皮细胞。它们在水稳态、酸碱控制、化合物的重吸收以及外源性物质和内源性代谢物的分泌中发挥重要作用。当暴露于缺血性、毒性、脓毒性或阻塞性条件时，它们的死亡导致我们临床上认为的急性肾损伤。此外，由于肾细胞有限的再生潜力，促纤维化环境不可避免地导致慢性肾脏疾病。在这篇综述中，我们将集中在人类近端肾小管上皮细胞。我们将涵盖人类肾脏培养模型，细胞来源，分离，培养，永生化和表征细分为形态，表型和功能表征。
The kidney is a complex organ, comprised primarily of glomerular, tubular, mesangial, and endothelial cells, and podocytes. The fact that renal cells are terminally differentiated at 34 weeks of gestation is the main obstacle in regeneration and treatment of acute kidney injury or chronic kidney disease. Furthermore, the number of chronic kidney disease patients is ever increasing and with it the medical community should aim to improve existing and develop new methods of renal replacement therapy. On the other hand, as polypharmacy is on the rise, thought should be given into developing new ways of testing drug safety. A possible way to tackle these issues is with isolation and culture of renal cells. Several protocols are currently described to isolate the desired cells, of which the most isolated are the proximal tubular epithelial cells. They play a major role in water homeostasis, acid–base control, reabsorption of compounds, and secretion of xenobiotics and endogenous metabolites. When exposed to ischemic, toxic, septic, or obstructive conditions their death results in what we clinically perceive as acute kidney injury. Additionally, due to renal cells' limited regenerative potential, the profibrotic environment inevitably leads to chronic kidney disease. In this review we will focus on human proximal tubular epithelial cells. We will cover human kidney culture models, cell sources, isolation, culture, immortalization, and characterization subdivided into morphological, phenotypical, and functional characterization.
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组织工程肾脏疾病模型。
影响因子：
16.1
作者：
DesRochers, Teresa M.;Palma, Erica;Kaplan, David L.
通讯作者：
Kaplan, David L.
影响因子：
5.8
作者：
Zhuo JL;Li XC
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Li XC
影响因子：
19.6
作者：
通讯作者：
--
影响因子：
0.7
作者：
Ferenbach DA;Bonventre JV
通讯作者：
Bonventre JV
DOI：
10.1016/s0022-2143(97)90180-3
发表时间：
1997-03-01
期刊：
JOURNAL OF LABORATORY AND CLINICAL MEDICINE
影响因子：
--
作者：
Racusen, LC;Monteil, C;Morin, JP
通讯作者：
Morin, JP