Identification of a novel chromosomal passenger complex and its unique localization during cytokinesis in Trypanosoma brucei.
Identification of a novel chromosomal passenger complex and its unique localization during cytokinesis in Trypanosoma brucei.
复制标题
DOI：
10.1371/journal.pone.0002354
复制
发表时间：
2008-06-11
期刊：
影响因子：
3.7
通讯作者：
Wang CC
中科院分区：
文献类型：
--
作者：
Li Z;Lee JH;Chu F;Burlingame AL;Günzl A;Wang CC
极光B激酶是染色体乘客复合物（CPC）的关键组成部分，它调节染色体分离和细胞质分裂。在布鲁氏锥虫（TbAUK1）中发现了Aurora B的同源物，但尚未发现该复合物的其他保守成分。本研究通过串联亲和纯化和质谱技术鉴定出4种新的TbAUK1相关蛋白。在这四种蛋白中，TbKIN-A和TbKIN-B是新的同源蛋白，而TbCPC1和TbCPC2是假设的蛋白，与酵母和后生动物中已知的CPC成分没有任何序列相似性。rna介导的四个基因的沉默导致纺锤体组装、染色体分离和细胞质分裂的丧失。在有丝分裂过程中，TbKIN-A定位于纺锤体，TbKIN-B定位于纺锤体中部，而TbCPC1、TbCPC2和TbAUK1则显示CPC的动态定位模式。有丝分裂后，CPC从中央纺锤体消失，重新定位于母细胞的背侧中点，在那里子细胞的前端被系住，开始向后端分裂，这表明真核生物中最不寻常的CPC启动的细胞分裂。
Aurora B kinase is a key component of the chromosomal passenger complex (CPC), which regulates chromosome segregation and cytokinesis. An ortholog of Aurora B was characterized in Trypanosoma brucei (TbAUK1), but other conserved components of the complex have not been found. Here we identified four novel TbAUK1 associated proteins by tandem affinity purification and mass spectrometry. Among these four proteins, TbKIN-A and TbKIN-B are novel kinesin homologs, whereas TbCPC1 and TbCPC2 are hypothetical proteins without any sequence similarity to those known CPC components from yeasts and metazoans. RNAi-mediated silencing of each of the four genes led to loss of spindle assembly, chromosome segregation and cytokinesis. TbKIN-A localizes to the mitotic spindle and TbKIN-B to the spindle midzone during mitosis, whereas TbCPC1, TbCPC2 and TbAUK1 display the dynamic localization pattern of a CPC. After mitosis, the CPC disappears from the central spindle and re-localizes at a dorsal mid-point of the mother cell, where the anterior tip of the daughter cell is tethered, to start cell division toward the posterior end, indicating a most unusual CPC-initiated cytokinesis in a eukaryote.
登录
查看更多内容
影响因子：
11.4
作者：
Kohl, L;Robinson, D;Bastin, P
通讯作者：
Bastin, P
影响因子：
3.3
作者：
Kwon, M;Morales-Mulia, S;Scholey, JM
通讯作者：
Scholey, JM
影响因子：
4.8
作者：
Hammarton, TC;Clark, J;Mottram, JC
通讯作者：
Mottram, JC
影响因子：
3.3
作者：
Bishop, JD;Han, ZB;Schumacher, JM
通讯作者：
Schumacher, JM
影响因子：
64.8
作者：
NISLOW, C;LOMBILLO, VA;MCINTOSH, JR
通讯作者：
MCINTOSH, JR