Rapid liquid chromatography-mass spectrometry quantitation of glucose-regulating hormones from human islets of Langerhans.
Rapid liquid chromatography-mass spectrometry quantitation of glucose-regulating hormones from human islets of Langerhans.
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朗格汉斯胰岛葡萄糖调节激素的快速液相色谱-质谱定量分析。
DOI：
10.1016/j.chroma.2020.461805
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发表时间：
2021-01-25
期刊：
影响因子：
--
通讯作者：
Roper MG
中科院分区：
文献类型：
--
作者：
Donohue MJ;Filla RT;Steyer DJ;Eaton WJ;Roper MG
葡萄糖稳态是通过胰岛分泌肽类激素来维持的，胰岛是胰腺内的内分泌细胞群，这些肽类激素包括胰岛素、生长抑素和胰高血糖素。本报告描述了一种LC-MS方法，使用多反应监测定量胰岛素，C-肽，胰高血糖素和生长抑素分泌的人胰岛细胞群。对于快速分析，使用2.1 × 30 mm（i.d.）×长度）C18色谱柱，核壳颗粒直径为2.7 μm。牺牲蛋白水解产物与样品一起使用，发现其可改善信号幅度、重复性并减少运行之间的残留。在优化的梯度条件下，梯度运行时间为4.55分钟，产生的平均峰宽为0.3分钟，最小分辨率为1.2，峰容量为20。作为概念验证，该方法用于测量来自2个供体的人胰岛静态孵育物的分泌物。胰岛素和C-肽进行了定量，并与这些激素的文献值匹配良好。我们期望这种无抗体定量胰岛分泌的多种激素将在未来提供对这些肽的时间关系的见解。
Glucose homeostasis is maintained through the secretion of peptide hormones, such as insulin, somatostatin, and glucagon, from islets of Langerhans, clusters of endocrine cells found in the pancreas. This report describes an LC-MS method using multiple reaction monitoring for quantitation of insulin, C-peptide, glucagon, and somatostatin secretion from human islet populations. For rapid analysis, a 5 min separation was achieved using a 2.1 × 30 mm (i.d. × length) C18 column with 2.7 μm diameter core shell particles. A sacrificial protein hydrolysate was used with the sample and found to improve signal magnitude, repeatability, and to reduce carryover between runs. At optimized gradient conditions, the gradient run time was 4.55 min producing an average peak width of 0.3 min, a minimum resolution of 1.2, and a peak capacity of 20. As a proof of concept, the method was used to measure secretions from static incubations of human islets from 2 donors. Insulin and C-peptide were quantified and matched well with literature values of these hormones. We expect that this antibody-free quantitation of multiple hormones secreted from islets will provide insights into the temporal relationships of these peptides in the future.
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影响因子：
4.1
作者：
Guillo, Christelle;Roper, Michael G.
通讯作者：
Roper, Michael G.
影响因子：
7.4
作者：
Lomasney, Anna R.;Yi, Lian;Roper, Michael G.
通讯作者：
Roper, Michael G.
影响因子：
4.1
作者：
Caslavska, J;Allemann, D;Thormann, W
通讯作者：
Thormann, W
DOI：
10.1152/ajpendo.00251.2015
发表时间：
2015-10-01
影响因子：
5.1
作者：
Henquin, Jean-Claude;Dufrane, Denis;Nenquin, Myriam
通讯作者：
Nenquin, Myriam
影响因子：
3.3
作者：
Fu, Zhuo;Gilbert, Elizabeth R.;Liu, Dongmin
通讯作者：
Liu, Dongmin