Ginsenoside Rb1 attenuates homocysteine-augmented guidewire injury-induced intimal hyperplasia in mice.
Ginsenoside Rb1 attenuates homocysteine-augmented guidewire injury-induced intimal hyperplasia in mice.
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DOI：
10.1016/j.jss.2008.07.005
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发表时间：
2009-12
期刊：
影响因子：
--
通讯作者：
Zhou W
中科院分区：
文献类型：
--
作者：
Chai H;Dong Y;Wang X;Zhou W
血管内膜增生(IH)是血管成形术后再狭窄的主要原因。本研究旨在探讨同型半胱氨酸(Hcy)和人参皂苷Rb1(Rb1)对高血压大鼠的影响。12周龄C57BL/6J小鼠左侧颈总动脉(CCA)用导丝剥离，右侧CCA作为未损伤对照。处死前分别给予生理盐水(NS)、Hcy、Rb1或Hcy+Rb1治疗4wk。分别于4、6、8周处死动物。取两个颈总动脉，计算内中膜厚度(IMT)比值。并对局部巨噬细胞的分布进行了研究。组织学分析显示，损伤的CCA节段持续存在内弹力板断裂和局灶性IH。IH的程度与损伤后的时间长短相关。Hcy治疗组IMT明显高于NS组(P<；0.05)，而Rb1组与NS组相似。Hcy+Rb1组IMT较Hcy组有显著改善(P<；0.01)。此外，与单独损伤或Rb1处理的动物相比，Hcy显著增加了局部巨噬细胞的含量。我们的研究表明，Hcy增加了损伤的CCA中IH的程度和巨噬细胞的含量，而Rb1减轻了这些不利影响。这些变化可能是通过Rb1的抗氧化作用来实现的。我们的数据提示人参在控制Hcy相关的血管损伤方面有潜在的临床应用。
Intimal hyperplasia (IH) is the primary cause for post-angioplasty restenosis. The purpose of this study is to investigate the effects of homocysteine (Hcy) and ginsenoside Rb1 (Rb1) on IH using a guidewire injury animal model. In 12-wk-old C57BL/6J mice, the left common carotid artery (CCA) was denudated with a guidewire and the right CCA was used as the uninjured control. They were treated with saline (NS), Hcy, Rb1, or Hcy + Rb1 for 4 wk prior to sacrifice. Animals were sacrificed at 4, 6, or 8 wk. Both CCAs were harvested and intimal-medium thickness (IMT) ratios were calculated. Local macrophage distribution was also studied. Histology analyses demonstrated consistent internal elastic lamina disruption and focal IH in the injured CCA segments. The degree of IH correlated to the lengths of time following injury. Hcy treated group had significant increase in IMT compared with the NS group (P < 0.05), while Rb1 group was similar to the NS group. In addition, Hcy + Rb1 group showed significant improvement in IMT compared with Hcy group (P < 0.01). Furthermore, Hcy significantly increased local macrophage content as compared with either lesion alone or Rb1 treated animals. Our study showed that Hcy increased the degree of IH and macrophage content in the injured CCA and that Rb1 attenuated these adverse effects. These changes might be mediated through antioxidative effects of Rb1. Our data suggests a potential clinical application of ginseng in controlling Hcy-related vascular injuries.
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