Response of isolated sperm plasma membranes from sea urchin to egg jelly.
Response of isolated sperm plasma membranes from sea urchin to egg jelly.
复制标题
海胆分离精子质膜对卵冻的反应。
DOI：
10.1111/j.1432-1033.1984.tb08496.x
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
I. Vargas
中科院分区：
文献类型：
--
作者：
A. Darszon;M. Gould;L. de De la Torre;I. Vargas
海胆精子的顶体反应是由卵子周围的糖蛋白胶冻诱导的，并伴随着精子质膜离子渗透性的变化。为了了解哪些膜成分参与了对果冻的反应，我们已经开始将精子膜成分重新组装成人造膜，并模拟精子中发生的渗透性变化进行分析。海水中的果冻在诱导顶体反应的浓度下不会显着改变仅用大豆脂质制成的超声处理的单层囊泡的 45Ca2+ 摄取（果冻：对照摄取的比率 = 1.08 +/- 0.36 SD，n = 21）。用大豆脂质或精子脂质提取物制成的纯脂质平面双层并在不同电压下进行的实验也没有显示在可比较的果冻浓度下有显着的渗透性变化。因此，果冻本身不会改变纯脂质双层的电导。相反，通过共声处理大豆磷脂制成的囊泡和通过 Cross, N. L. [1983, J. Cell Sci. [1983, J. Cell Sci. 59, 13-25]（果冻比例：对照摄取 = 1.51 +/- 0.75，n = 20，20 个实验中 16 个呈阳性）。混合囊泡的钙吸收反应也是物种特异性的：点状Arbacia jelly不会出现这种情况（Arbacia jelly：对照的比率= 1.18 +/- 0.51；Strongylocentrotus jelly：control的比率= 1.71 +/- 0.97，n = 10；P----0.025，配对t统计）。用大豆脂质和精子膜辛基葡萄糖苷提取物制成的囊泡也对果冻有反应，增加了 45Ca2+ 的吸收。我们的结果表明，我们具备分离和表征参与卵冻诱导的通透性变化的精子膜成分的起始条件。
The acrosome reaction in sea urchin sperm is induced by a glycoprotein jelly surrounding the egg and is accompanied by changes in ion permeability of sperm plasma membrane. In an attempt to learn what membrane components are involved in the response to jelly, we have begun to reassemble sperm membrane components into artificial membranes and assay for permeability changes mimicking those that occur in sperm. Jelly in sea water at concentrations that induce the acrosome reaction did not significantly change 45Ca2+ uptake of sonicated unilamellar vesicles made with soybean lipid only (ratio jelly:control uptake = 1.08 +/- 0.36 SD, n = 21). Experiments with pure lipid planar bilayers made with soybean lipid or a lipid extract from sperm and held at various voltages, also did not reveal substantial permeability changes at comparable jelly concentrations. Thus, jelly by itself does not change the conductance of a pure lipid bilayer. In contrast, significant (P----0.0005, t test for two sample means) 45Ca2+ uptake was observed with vesicles made by cosonicating soybean phospholipids and Strongylocentrotus purpuratus sperm membranes isolated by the method of Cross, N. L. [1983, J. Cell Sci. 59, 13-25] (ratio jelly: control uptake = 1.51 +/- 0.75, n = 20, 16 positive out of 20 experiments). The calcium uptake response of the mixed vesicles was also species-specific: it did not occur with jelly from Arbacia punctulata (ratio Arbacia jelly: control = 1.18 +/- 0.51; ratio Strongylocentrotus jelly: control = 1.71 +/- 0.97, n = 10; P----0.025, paired t statistic). Vesicles made with soybean lipid and an octyl glucoside extract of sperm membranes also responded to jelly with increased 45Ca2+ uptake. Our results indicate that we have the starting conditions to isolate and characterize the sperm membrane components that participate in the egg jelly induced permeability changes.
精子表面糖蛋白抗体抑制卵冻诱导的海胆精子顶体反应。
影响因子：
2.7
作者：
Lopo,AC;Vacquier,VD
通讯作者：
Vacquier,VD