Inner retinal inhibition shapes the receptive field of retinal ganglion cells in primate.
Inner retinal inhibition shapes the receptive field of retinal ganglion cells in primate.
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DOI：
10.1113/jphysiol.2013.257352
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发表时间：
2014-01-01
期刊：
影响因子：
--
通讯作者：
Solomon SG
中科院分区：
文献类型：
--
作者：
Protti DA;Di Marco S;Huang JY;Vonhoff CR;Nguyen V;Solomon SG
摘要感受野的中心-周围结构是大多数视网膜神经节细胞（RGCs）的一个特征，对于空间辨别和对比度检测至关重要。 虽然已知侧抑制过程在产生感受野环绕中是重要的，但灵长类动物视网膜中两个突触层中的每一个的贡献仍然不清楚。在这里，我们研究了灵长类动物视网膜上的ON和OFF神经节细胞的兴奋性和抑制性突触输入的空间组织。所有的RGC表现出增加的兴奋响应刺激的首选极性。对RGC的抑制包括两种类型的反应，以首选极性：一些RGC表现出抑制的增加，而其他人则表现出消除紧张性抑制。兴奋性输入强烈的空间调谐，但抑制性输入表现出更多变的组织：在一些神经元，他们强烈调整的兴奋，在其他抑制性输入显示没有空间调谐。我们针对一个来源的内部视网膜抑制功能性消融尖峰无长突细胞浴应用河豚毒素（TTX）。TTX显着减少兴奋性输入的空间调谐。此外，TTX减少对那些RGC的抑制，其中优选极性的刺激增加抑制。通过体细胞注射兴奋性和抑制性突触电导的空间调谐特性的重建验证了对双极细胞的TTX介导的抑制增加了RGC尖峰输出中周围的强度。这些结果表明，在灵长类动物的视网膜内丛状层的抑制机制锐化神经节细胞的空间调谐。
Abstract The centre–surround organisation of receptive fields is a feature of most retinal ganglion cells (RGCs) and is critical for spatial discrimination and contrast detection. Although lateral inhibitory processes are known to be important in generating the receptive field surround, the contribution of each of the two synaptic layers in the primate retina remains unclear. Here we studied the spatial organisation of excitatory and inhibitory synaptic inputs onto ON and OFF ganglion cells in the primate retina. All RGCs showed an increase in excitation in response to stimulus of preferred polarity. Inhibition onto RGCs comprised two types of responses to preferred polarity: some RGCs showed an increase in inhibition whilst others showed removal of tonic inhibition. Excitatory inputs were strongly spatially tuned but inhibitory inputs showed more variable organisation: in some neurons they were as strongly tuned as excitation, and in others inhibitory inputs showed no spatial tuning. We targeted one source of inner retinal inhibition by functionally ablating spiking amacrine cells with bath application of tetrodotoxin (TTX). TTX significantly reduced the spatial tuning of excitatory inputs. In addition, TTX reduced inhibition onto those RGCs where a stimulus of preferred polarity increased inhibition. Reconstruction of the spatial tuning properties by somatic injection of excitatory and inhibitory synaptic conductances verified that TTX-mediated inhibition onto bipolar cells increases the strength of the surround in RGC spiking output. These results indicate that in the primate retina inhibitory mechanisms in the inner plexiform layer sharpen the spatial tuning of ganglion cells.
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DOI：
10.1523/jneurosci.1218-09.2009
发表时间：
2009-07-01
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Crook JD;Davenport CM;Peterson BB;Packer OS;Detwiler PB;Dacey DM
通讯作者：
Dacey DM
影响因子：
2.5
作者：
Camp, Aaron J.;Cheong, Soon K.;Solomon, Samuel G.
通讯作者：
Solomon, Samuel G.
影响因子：
2.5
作者：
Bloomfield, SA
通讯作者：
Bloomfield, SA
影响因子：
16.2
作者：
Alitto, Henry J.;Usrey, W. Martin
通讯作者：
Usrey, W. Martin
影响因子：
5.5
作者：
CALDWELL, JH;DAW, NW
通讯作者：
DAW, NW