RNase E cleavage shapes the transcriptome of Rhodobacter sphaeroides and strongly impacts phototrophic growth.
RNase E cleavage shapes the transcriptome of Rhodobacter sphaeroides and strongly impacts phototrophic growth.
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DOI：
10.26508/lsa.201800080
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发表时间：
2018-08
影响因子：
4.4
通讯作者：
Klug G
中科院分区：
文献类型：
--
作者：
Förstner KU;Reuscher CM;Haberzettl K;Weber L;Klug G
本研究确定了球形红杆菌转录组中内切核糖核酸酶RNase E的裂解位点，并证明了其在抗氧化性和光营养生长中的作用。细菌通过其转录组的快速变化来适应不断变化的环境条件。这不仅是通过调节转录速度实现的，也是通过处理和降解RNA实现的。我们应用TIER-SEQ(瞬时失活内切核酸酶后RNA-SEQ)在转录组范围内鉴定RNase E裂解位点和5‘RNA端，当RNase E活性在球形红杆菌中降低时，它们会被丰富。这些结果揭示了RNaseE对于这种富含鸟嘌呤胞嘧啶的α蛋白细菌中mRNAs、rRNAs和sRNAs的成熟和周转的重要性，后者中的一些在氧化应激反应中具有被很好地描述的功能。与此一致，核糖核酸酶E在氧化应激反应中的作用被证明。在光合作用复合体的形成和光营养生长方面，观察到一株RNase E活性降低的突变体的表型非常强，而对化学营养生长没有影响。
This study identifies the cleavage sites of the endoribonuclease RNase E in the Rhodobacter sphaeroides transcriptome and demonstrates its effect on oxidative stress resistance and phototrophic growth. Bacteria adapt to changing environmental conditions by rapid changes in their transcriptome. This is achieved not only by adjusting rates of transcription but also by processing and degradation of RNAs. We applied TIER-Seq (transiently inactivating an endoribonuclease followed by RNA-Seq) for the transcriptome-wide identification of RNase E cleavage sites and of 5′ RNA ends, which are enriched when RNase E activity is reduced in Rhodobacter sphaeroides. These results reveal the importance of RNase E for the maturation and turnover of mRNAs, rRNAs, and sRNAs in this guanine-cytosine-rich α-proteobacterium, some of the latter have well-described functions in the oxidative stress response. In agreement with this, a role of RNase E in the oxidative stress response is demonstrated. A remarkably strong phenotype of a mutant with reduced RNase E activity was observed regarding the formation of photosynthetic complexes and phototrophic growth, whereas there was no effect on chemotrophic growth.
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影响因子：
4.1
作者：
Baumgardt K;Šmídová K;Rahn H;Lochnit G;Robledo M;Evguenieva-Hackenberg E
通讯作者：
Evguenieva-Hackenberg E
影响因子：
4.4
作者：
Glaeser, Jens;Zobawa, Monica;Klug, Gabriele
通讯作者：
Klug, Gabriele
影响因子：
3.6
作者：
FRITSCH, J;ROTHFUCHS, R;KLUG, G
通讯作者：
KLUG, G
模因套件：用于发现和搜索的工具。
影响因子：
14.9
作者：
Bailey TL;Boden M;Buske FA;Frith M;Grant CE;Clementi L;Ren J;Li WW;Noble WS
通讯作者：
Noble WS
影响因子：
2.8
作者：
Baumgardt, Kathrin;Melior, Hendrik;Evguenieva-Hackenberg, Elena
通讯作者：
Evguenieva-Hackenberg, Elena