A comparison of the response of diploid and triploid Atlantic salmon (Salmo salar) siblings to a commercial furunculosis vaccine and subsequent experimental infection with Aeromonas salmonicida.
A comparison of the response of diploid and triploid Atlantic salmon (Salmo salar) siblings to a commercial furunculosis vaccine and subsequent experimental infection with Aeromonas salmonicida.
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DOI：
10.1016/j.fsi.2016.08.049
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发表时间：
2016-10
影响因子：
4.7
通讯作者：
Adams, Alexandra
中科院分区：
文献类型：
--
作者：
Chalmers, Lynn;Thompson, Kim D.;Taylor, John F.;Black, Sean;Migaud, Herve;North, Ben;Adams, Alexandra
不育三倍体鱼是解决水产养殖中性成熟和脱逃问题的一种方法。然而，由于疾病爆发继续给该行业造成重大经济损失，必须确定三倍体对疾病和疾病治疗的反应。本研究的目的是比较三倍体大西洋鲑鱼和二倍体鱼类对商品化真菌病疫苗的反应，并通过实验感染沙门氏气单胞菌来评估疫苗对两倍体的有效性。将二倍体和三倍体大西洋鲑鱼腹腔注射磷酸盐缓冲盐水、液体石蜡佐剂或商业真菌病疫苗。接种疫苗后，测量生长、粘附评分和各种评估免疫功能的测定，如呼吸爆发和抗体反应。750°d时，接种疫苗对攻毒前两倍体的体重均无显著影响。虽然没有观察到倍性的影响，但接种疫苗的鱼的粘附分数明显高于未接种疫苗的鱼。然而，接种疫苗后，倍性显著影响呼吸爆发活动，三倍体比二倍体表现出更高的活动。结合在三倍体中观察到的较低的白细胞数量，可能是这种低的细胞数量被增加的细胞活性所补偿。然而，倍性对补体活性或抗体反应没有显著影响，与未接种疫苗的对照相比，所有接种疫苗的鱼检测到的抗体水平明显更高。此外，两个倍性组在攻毒后都得到了很好的保护，相对存活率没有差异。基于这些结果，似乎倍性不影响疫苗接种后粘连的严重程度，也不影响疫苗接种后的免疫反应，而且疣病疫苗在二倍体和三倍体大西洋鲑鱼中都表现良好。商品糠疹疫苗对三倍体大西洋鲑鱼的保护效果。倍性不影响接种鱼黏附的严重程度。免疫应答的差异可能与三倍体的代偿机制有关。
Sterile triploid fish represent a solution to the problems associated with sexual maturation and escapees in aquaculture. However, as disease outbreaks continue to cause significant economic losses to the industry, it is essential that the response of triploids to disease and disease treatments be characterised. The aim of this study was to compare the response of triploid Atlantic salmon to a commercial furunculosis vaccine with that of diploid fish, and to assess the vaccine efficacy in the two ploidies through an experimental infection with Aeromonas salmonicida. Diploid and triploid Atlantic salmon were injected intraperitoneally with either phosphate buffered saline, liquid paraffin adjuvant or a commercial furunculosis vaccine. Following vaccination, growth, adhesion scores and a variety of assays to assess immune function, such as respiratory burst and antibody response, were measured. Vaccination did not have a significant effect on the weight of either ploidy prior to challenge at 750° days. Adhesion scores were significantly higher in vaccinated fish compared to unvaccinated fish, although no effect of ploidy was observed. Ploidy significantly affected respiratory burst activity following vaccination, however, with triploids exhibiting higher activity than diploids. Combined with lower white blood cell numbers observed in the triploids, it may be that this low cell number is compensated for by increased cellular activity. Ploidy however, did not have a significant effect on complement activity or antibody response, with significantly higher antibody levels detected in all vaccinated fish compared to unvaccinated controls. In addition, both ploidy groups were well protected following challenge with no difference in the relative percentage survival. Based on these results, it appears that ploidy does not affect the severity of adhesions that result post-vaccinate or in the fish's immune response following vaccination, and the furunculosis vaccine performs equally well in both diploid and triploid Atlantic salmon. Commercial furunculosis vaccine effective in protecting triploid Atlantic salmon. Ploidy did not affect the severity of the adhesions occurring in vaccinated fish. Differences in immune response may due to compensatory mechanism in triploids.
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影响因子：
4.7
作者：
Langston, AL;Johnstone, R;Ellis, AE
通讯作者：
Ellis, AE
影响因子：
--
作者：
Fraser, Thomas W. K.;Fjelldal, Per Gunnar;Mayer, Ian
通讯作者：
Mayer, Ian
影响因子：
4.5
作者：
Friars, GW;McMillan, I;Benfey, TJ
通讯作者：
Benfey, TJ
影响因子：
4.5
作者：
Leclercq, E.;Taylor, J. F.;Migaud, H.
通讯作者：
Migaud, H.
影响因子：
2.5
作者：
Fraser, T. W. K.;Mayer, I.;Fjelldal, P. G.
通讯作者：
Fjelldal, P. G.