Lipopolysaccharides (LPS) of Oral Black-Pigmented Bacteria Induce Tumor Necrosis Factor Production by LPS-Refractory C3H/HeJ Macrophages in a Way Different from That of Salmonella LPS
Lipopolysaccharides (LPS) of Oral Black-Pigmented Bacteria Induce Tumor Necrosis Factor Production by LPS-Refractory C3H/HeJ Macrophages in a Way Different from That of Salmonella LPS
复制标题
口腔黑色素细菌的脂多糖 (LPS) 以与沙门氏菌 LPS 不同的方式诱导 LPS 难治性 C3H/HeJ 巨噬细胞产生肿瘤坏死因子
作者：
T. Kirikae;T. Nitta;F. Kirikae;Y. Suda;S. Kusumoto;Nirofer Qureshi;M. Nakano
肠杆菌科S型或R型成员和口腔黑色素细菌（牙龈卟啉单胞菌和中间普雷沃氏菌）的脂多糖（LPS）制剂已知可激活LPS难治性C3 H/HeJ巨噬细胞。然而，当通过再纯化从肠杆菌科的R型LPS中去除污染蛋白时，这种能力就丧失了。在本研究中，我们研究了来自牙龈卟啉单胞菌、中间卟啉单胞菌、沙门氏菌和马流产沙门氏菌的LPS在γ-干扰素致敏的C3 H/HeJ巨噬细胞再纯化前后诱导肿瘤坏死因子（TNF）产生的能力。通过离心分配色谱法将马流产疫霉S-LPS分级分离成两种LPS形式：具有同源长O-多糖链的SL-LPS和具有短寡糖链的SS-LPS。在再纯化之前，除了SL-LPS之外的所有LPS形式在C3 H/HeJ和C3 H/HeN巨噬细胞中诱导TNF产生。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示，再纯化去除了制剂中的污染蛋白，并再纯化了SS-LPS和S。在C3 H/HeJ巨噬细胞中，尽管C3 H/HeN巨噬细胞仍保持响应性，但min Ra-LPS不再刺激TNF产生。相反，再纯化的口腔细菌LPS保留了诱导C3 H/HeJ巨噬细胞产生TNF的能力。口服细菌LPS制剂也没有拮抗过量的无活性，再纯化的SL-LPS; Ra-LPS; Rhodobacter sphaeroides lipid A，一种竞争性LPS拮抗剂，或紫杉醇，一种LPS激动剂，他们是比较耐多粘菌素B治疗。然而，口服细菌LPS对D-半乳糖胺处理的C3 H/HeN小鼠的毒性低于沙门氏菌LPS。提示牙龈卟啉单胞菌和中间型卟啉单胞菌LPS的活性分子和作用方式与沙门氏菌LPS不同。
ABSTRACT Some lipopolysaccharide (LPS) preparations from S- or R-form members of the family Enterobacteriaceae and oral black-pigmented bacteria (Porphyromonas gingivalis andPrevotella intermedia) are known to activate LPS-refractory C3H/HeJ macrophages. When contaminating proteins are removed from R-form LPS ofEnterobacteriaceae by repurification, however, this ability is lost. In the present study, we investigated the capacity of LPS from P. gingivalis, P. intermedia,Salmonella minnesota, and Salmonella abortusequi to induce production of tumor necrosis factor (TNF) in gamma interferon-primed C3H/HeJ macrophages before and after repurification. P. abortusequi S-LPS was fractionated by centrifugal partition chromatography into two LPS forms: SL-LPS, having homologous long O-polysaccharide chains, and SS-LPS having short oligosaccharide chains. Prior to repurification, all LPS forms except SL-LPS induced TNF production in both C3H/HeJ and C3H/HeN macrophages. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that repurification removed contaminating protein from the preparations, and repurified SS-LPS and S. minnesota Ra-LPS no longer stimulated TNF production in C3H/HeJ macrophages, although C3H/HeN macrophages remained responsive. In contrast, repurified oral bacterial LPS retained the capacity to induce TNF production in C3H/HeJ macrophages. Oral bacterial LPS preparations also were not antagonized by excess inactive, repurified SL-LPS; Ra-LPS; Rhodobacter sphaeroides lipid A, a competitive LPS antagonist, or paclitaxel, an LPS agonist, and they were comparatively resistant to polymyxin B treatment. Nevertheless, oral bacterial LPS was less toxic tod-galactosamine-treated C3H/HeN mice than was LPS fromSalmonella. These findings indicate that the active molecule(s) and mode of action of LPS from P. gingivalisand P. intermedia are quite different from those of LPS from Salmonella.
登录
查看更多内容
影响因子：
56.9
作者：
DING, AH;PORTEU, F;NATHAN, CF
通讯作者：
NATHAN, CF
影响因子：
4.4
作者：
C. Amura;L. C. Chen;N. Hirohashi;M. Lei;D. Morrison
通讯作者：
C. Amura;L. C. Chen;N. Hirohashi;M. Lei;D. Morrison
球形红假单胞菌脂多糖中二磷酰脂质 A 脂肪酸中 3-氧代和不饱和基团的化学还原。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Qureshi,N;Takayama,K;Meyer,KC;Kirkland,TN;Bush,CA;Chen,L;Wang,R;Cotter,RJ
通讯作者：
Cotter,RJ
牙龈拟杆菌菌株 W50、W83 和 ATCC 33277 脂多糖的化学特征和生物学特性。
影响因子：
--
作者：
Bramanti,TE;Wong,GG;Weintraub,ST;Holt,SC
通讯作者：
Holt,SC
DOI：
10.1016/s0021-9258(18)55023-7
发表时间：
1991-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Douglas T. GolenbockSQll;Randolph;HamptonIIQ;N. Qureshi;K. Takayama;Christian R. H. RaetzQ
通讯作者：
Douglas T. GolenbockSQll;Randolph;HamptonIIQ;N. Qureshi;K. Takayama;Christian R. H. RaetzQ