The effect of laser-assisted hatching on the methylation and expression pattern of imprinted gene IGF2/H19 in mouse blastocysts and offspring
The effect of laser-assisted hatching on the methylation and expression pattern of imprinted gene IGF2/H19 in mouse blastocysts and offspring
复制标题
激光辅助孵化对小鼠囊胚及子代印迹基因IGF2/H19甲基化及表达模式的影响
DOI：
10.1007/s10815-020-01975-4
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发表时间：
2020-10
影响因子：
3.1
通讯作者：
Zhang Shun
中科院分区：
文献类型：
--
作者：
Huo Peng;Deng Kai;Wang Lulu;Li Man;Yao Jun;Le Jianghua;Lei Xiaocan;Zhang Shun
目的 本研究旨在探讨激光辅助孵化打孔和减薄处理对胚胎及子代印迹基因IGF2/H19表达和甲基化的影响。方法将经过打孔或减薄处理的预孵化囊胚或对照预孵化囊胚移植到代孕子宫中。使用亚硫酸氢盐转化介导的测序和实时PCR分别评估IGF2/H19印记控制区（ICR）的DNA甲基化以及IGF2和H19的表达。结果 与对照组和减薄组相比，钻孔组的孵化囊胚发育率显着增加。间伐组孵化囊胚的 IGF2/H19 ICR DNA 甲基化水平为 27.33%，而对照组和钻孔组观察到的 IGF2/H19 ICR DNA 甲基化水平为 38.67% 和 36%。与对照组和钻孔组相比，稀疏处理增加了胎盘中 IGF2/H19 ICR 的 DNA 甲基化水平。钻孔和间伐处理降低了预孵化、已孵化囊胚和胎盘中H19 mRNA的表达水平，而间伐组子代H19 mRNA的表达水平显着增加。间苗处理使孵化前囊胚和子代IGF2 mRNA表达水平升高，胎盘显着下降；而打孔处理则使孵化囊胚和胎盘IGF2 mRNA表达水平显着升高。结论这些观察结果表明，用于体外培养小鼠囊胚孵化的钻孔可以提高后代的产量。
Purpose This study aimed to determine the effects of drilling and thinning treatment of laser-assisted hatching on the expression and methylation of imprinted gene IGF2/H19 in embryos and offspring. Methods The prehatching blastocysts with treatment of drilling or thinning, or control prehatching blastocysts, were transplanted in surrogate uteri. The DNA methylation of IGF2/H19 imprinting control region (ICR) and the expression of IGF2 and H19 were respectively evaluated using bisulfite conversion-mediated sequencing and real-time PCR. Results The drilling group showed a significant increase in the development rate of hatched blastocysts in comparison with the control and thinning group. DNA methylation level of IGF2/H19 ICR of hatched blastocysts in the thinning group was 27.33% in comparison with the 38.67% and 36% observed in the control and drilling group. The thinning treatment increased the DNA methylation level of IGF2/H19 ICR in the placenta in comparison with the control and drilling group. The drilling and thinning treatment decreased the expression level of H19 mRNA in prehatching and hatched blastocysts as well as placenta, while a significant increase in the expression level of H19 mRNA of offspring was observed in the thinning group. The thinning treatment increased the expression level of IGF2 mRNA of prehatching blastocysts and offspring and a significant decrease in placenta, while the drilling treatment resulted in a significant increase in the expression level of IGF2 mRNA of hatched blastocysts and placenta. Conclusion These observations suggested that drilling used for hatching of in vitro cultured mouse blastocysts may improve the production of offspring.
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影响因子：
--
作者：
G. Koustas;C. Sjoblom
通讯作者：
G. Koustas;C. Sjoblom
影响因子：
2.3
作者：
En-Hua Wang;An-Cong Wang;Bao-Song Wang;Bin Li
通讯作者：
En-Hua Wang;An-Cong Wang;Bao-Song Wang;Bin Li
影响因子：
2
作者：
Cocchi, Guido;Marsico, Concetta;Cosentino, Anita;Spadoni, Chiara;Rocca, Alessandro;De Crescenzo, Agostina;Riccio, Andrea
通讯作者：
Riccio, Andrea
八字形孵化增加了小鼠胚胎长期培养中内细胞团分裂的风险
影响因子：
3.7
作者：
Kuang, Yanping;Wu, Lingqian;Lu, Shengsheng;Lyu, Qifeng
通讯作者：
Lyu, Qifeng
影响因子：
2.5
作者：
COHEN, J;FELDBERG, D
通讯作者：
FELDBERG, D