Extracellular RNA: mechanisms of secretion and potential functions.
Extracellular RNA: mechanisms of secretion and potential functions.
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DOI：
10.1093/jxb/erac512
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发表时间：
2023-04-09
影响因子：
6.9
通讯作者：
中科院分区：
文献类型：
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作者：
长期以来，细胞外RNA（exRNA）一直被视为植物可降解并用于循环利用营养物质的细胞废弃物。然而，近期研究结果表明，有必要重新审视植物细胞外RNA的生物学意义。一些研究显示，细胞外RNA种类繁多，实则是一组极为多样的非编码RNA，其组成种类长度从十几个核苷酸到数百个核苷酸不等。它们大多以游离形式存在，或与RNA结合蛋白相关联，而极少存在于细胞外囊泡（EVs）内。尽管与细胞外囊泡相关的小RNA含量较低，但因其在介导跨界RNA干扰中的潜在作用，一直是细胞外RNA研究的焦点。因此，直到最近，非囊泡细胞外RNA才开始受到关注。在此，我们总结了目前对构成细胞外RNA组的RNA种类的认知，并探讨了能够解释细胞外RNA多样性的机制，不仅聚焦于RNA分泌可能涉及的潜在机制，还关注细胞外RNA释放后的加工过程。我们还将分享关于囊泡和非囊泡细胞外RNA在植物与病原体相互作用、细胞间通讯以及植物其他生理过程中潜在作用的观点。
在这篇综述中，我们探讨了植物细胞外RNA的生物学意义，并对其在质外体中的分泌、加工和运输提供了见解。
Extracellular RNA (exRNA) has long been considered as cellular waste that plants can degrade and utilize to recycle nutrients. However, recent findings highlight the need to reconsider the biological significance of RNAs found outside of plant cells. A handful of studies suggest that the exRNA repertoire, which turns out to be an extremely heterogenous group of non-coding RNAs, comprises species as small as a dozen nucleotides to hundreds of nucleotides long. They are found mostly in free form or associated with RNA-binding proteins, while very few are found inside extracellular vesicles (EVs). Despite their low abundance, small RNAs associated with EVs have been a focus of exRNA research due to their putative role in mediating trans-kingdom RNAi. Therefore, non-vesicular exRNAs have remained completely under the radar until very recently. Here we summarize our current knowledge of the RNA species that constitute the extracellular RNAome and discuss mechanisms that could explain the diversity of exRNAs, focusing not only on the potential mechanisms involved in RNA secretion but also on post-release processing of exRNAs. We will also share our thoughts on the putative roles of vesicular and extravesicular exRNAs in plant–pathogen interactions, intercellular communication, and other physiological processes in plants. In this review, we discuss the biological significance of extracellular RNAs in plants and provide insights into their secretion, processing, and trafficking within the apoplast.
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影响因子：
4.5
作者：
Albanese M;Chen YA;Hüls C;Gärtner K;Tagawa T;Mejias-Perez E;Keppler OT;Göbel C;Zeidler R;Shein M;Schütz AK;Hammerschmidt W
通讯作者：
Hammerschmidt W
影响因子：
11.8
作者：
Barman, Bahnisikha;Sung, Bong Hwan;Krystofiak, Evan;Ping, Jie;Ramirez, Marisol;Millis, Bryan;Allen, Ryan;Prasad, Nripesh;Chetyrkin, Sergei;Calcutt, M. Wade;Vickers, Kasey;Patton, James G.;Liu, Qi;Weaver, Alissa M.
通讯作者：
Weaver, Alissa M.
影响因子：
4.9
作者：
Alexandersson, E;Saalbach, G;Kjellbom, P
通讯作者：
Kjellbom, P
DOI：
10.1126/science.aar4142
发表时间：
2018-06-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Cai Q;Qiao L;Wang M;He B;Lin FM;Palmquist J;Huang SD;Jin H
通讯作者：
Jin H
影响因子：
3.5
作者：
Cheng, Wei;Lin, Menglan;He, Shuilin
通讯作者：
He, Shuilin