Circular RNA VRK1 facilitates pre-eclampsia progression via sponging miR-221-3P to regulate PTEN/Akt.
Circular RNA VRK1 facilitates pre-eclampsia progression via sponging miR-221-3P to regulate PTEN/Akt.
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环状 RNA VRK1 通过海绵 miR-221-3P 调节 PTEN/Akt 促进先兆子痫进展
DOI：
10.1111/jcmm.16454
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发表时间：
2022-03
影响因子：
5.3
通讯作者：
Meng T
中科院分区：
文献类型：
--
作者：
Li Z;Zhou X;Gao W;Sun M;Chen H;Meng T
先兆子痫（PE）是一种全球性的妊娠相关疾病。其主要特征是滋养层细胞的缺陷迁移和侵袭。近年来，人们认为环状RNA（circular RNA，circRNA）在PE中起着重要作用。circRNA在PE中的表达模式和生物学功能仍然难以捉摸。在这里，我们进行了一个circRNA微阵列，以确定推定的PE相关的circRNA。生物信息学分析用于筛选与先兆子痫有潜在关系的circRNA，我们鉴定了一种在PE胎盘组织中上调的新型circRNA（circVRK1）。通过使用HTR-8/SVneo细胞，circVRK1敲低显著增强了细胞迁移和侵袭能力，以及上皮-间质转化（EMT）。从机制上讲，我们发现circVRK1和PTEN可以作为miR-221 - 3p的ceRNA发挥作用。miR-221 - 3p过表达通过调控PTEN促进细胞迁移、侵袭和EMT。miR-221 - 3p抑制剂或PTEN的共转染逆转了circVRK1敲低的作用。此外，circVRK1/miR-221 - 3p/PTEN轴极大地调节Akt磷酸化。一般来说，circVRK1通过作为miR-221 - 3p的ceRNA调节PTEN，进而抑制PI3K/Akt活化，从而抑制滋养层细胞迁移、侵袭和EMT。本文的目的是打开广泛的见解，以调查PE的发病，并提供新的潜在的治疗目标，在PE。
Pre‐eclampsia (PE) is a worldwide pregnancy‐related disorder. It is mainly characterized by defect migration and invasion of trophoblast cells. Recently, circular RNAs (circRNAs) have been believed to play a vital role in PE. The expression patterns and the biological functions of circRNAs in PE remain elusive. Here, we performed a circRNA microarray to identify putative PE‐related circRNAs. Bioinformatics analyses were used to screen the circRNAs which have potential relationships with pre‐eclampsia, and we identified a novel circRNA (circVRK1) that was up‐regulated in PE placenta tissues. By using HTR‐8/SVneo cells, circVRK1 knockdown significantly enhanced cell migration and invasion abilities, as well as epithelial‐mesenchymal transition (EMT). Mechanistically, we found that circVRK1 and PTEN could function as the ceRNAs to miR‐221‐3p. Overexpression of miR‐221‐3p promoted cell migration, invasion and EMT via regulating PTEN. The cotransfection of miR‐221‐3p inhibitor or PTEN reversed the effect from circVRK1 knockdown. Moreover, the circVRK1/miR‐221‐3p/PTEN axis greatly regulated Akt phosphorylation. In general, circVRK1 suppresses trophoblast cell migration, invasion and EMT, by acting as a ceRNA to miR‐221‐3p to regulate PTEN, and further inhibit PI3K/Akt activation. The purpose of this paper is to open wide insights to investigate the onset of PE and provide new potential therapeutic targets in PE.
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影响因子：
5.8
作者：
Kohnoh T;Hashimoto N;Ando A;Sakamoto K;Miyazaki S;Aoyama D;Kusunose M;Kimura M;Omote N;Imaizumi K;Kawabe T;Hasegawa Y
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作者：
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通讯作者：
Lazo, Pedro A.
DOI：
10.1007/s00018-018-2811-2
发表时间：
2018-07
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
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作者：
Campillo-Marcos I;Lazo PA
通讯作者：
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影响因子：
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作者：
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