Conjunctival reconstruction with progenitor cell-derived autologous epidermal sheets in rhesus monkey.
Conjunctival reconstruction with progenitor cell-derived autologous epidermal sheets in rhesus monkey.
复制标题
用祖细胞衍生的自体表皮片重建恒河猴的结膜
DOI：
10.1371/journal.pone.0025713
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Ge J
中科院分区：
文献类型：
--
作者：
Lu R;Zhang X;Huang D;Huang B;Gao N;Wang Z;Ge J
严重的眼表疾病是当今临床医生面临的最具挑战性的问题之一。传统的治疗通常不令人满意，这些疾病的长期眼部后果是毁灭性的。寻找结膜上皮细胞的替代品具有重要意义。本研究旨在探讨在脱落的羊膜上利用祖细胞来源的表皮片重建结膜损伤猴眼表的可能性。采用IV型胶原黏附法分离恒河猴表皮祖细胞，体外在剥去的羊膜上扩增祖细胞源性表皮片。在结膜损伤后3周，采用自体培养的表皮祖细胞移植重建4只猴子受损的眼表。术后2周切除移植物，采用苏木精-伊红染色、周期性酸希夫染色、免疫荧光染色、扫描电镜和透射电镜检查。移植片的组织学检查显示，细胞片健康存活，与脱落的羊膜粘附良好，有多层上皮细胞。电镜显示上皮细胞外观与正常结膜上皮非常相似，未见杯状细胞。移植的上皮细胞有大量的桥粒连接，并以半桥粒附着在羊膜上。免疫组化证实移植的表皮祖细胞中存在结膜特异性标志物粘蛋白4和角蛋白4。综上所述，我们的研究成功地将祖细胞来源的表皮片移植到脱落的AM上重建了结膜损伤猴子的结膜，这是评估非眼表源祖细胞自体移植应用的第一步。表皮祖细胞可作为结膜上皮细胞的新替代品，克服自体结膜缺乏的问题。
Severe ocular surface diseases are some of the most challenging problems that the clinician faces today. Conventional management is generally unsatisfactory, and the long-term ocular consequences of these conditions are devastating. It is significantly important to find a substitute for conjunctival epithelial cells. This study was to explore the possibility of progenitor cell-derived epidermal sheets on denuded amniotic membrane to reconstruct ocular surface of conjunctiva damaged monkeys. We isolated epidermal progenitor cells of rhesus monkeys by type IV collagen adhesion, and then expanded progenitor cell-derived epidermal sheets on denuded amniotic membrane ex vivo. At 3 weeks after the conjunctiva injury, the damaged ocular surface of four monkeys was surgically reconstructed by transplanting the autologous cultivated epidermal progenitor cells. At 2 weeks after surgery, transplants were removed and examined with Hematoxylin-eosin staining, Periodic acid Schiff staining, immunofluorescent staining, scanning and transmission electron microscopy. Histological examination of transplanted sheets revealed that the cell sheets were healthy alive, adhered well to the denuded amniotic membrane, and had several layers of epithelial cells. Electron microscopy showed that the epithelial cells were very similar in appearance to those of normal conjunctival epithelium, even without goblet cell detected. Epithelial cells of transplants had numerous desmosomal junctions and were attached to the amniotic membrane with hemidesmosomes. Immunohistochemistry confirmed the presence of the conjunctival specific markers, mucin 4 and keratin 4, in the transplanted epidermal progenitor cells. In conclusion, our present study successfully reconstructed conjunctiva with autologous transplantation of progenitor cell-derived epidermal sheets on denuded AM in conjunctival damaged monkeys, which is the first step toward assessing the use of autologous transplantation of progenitor cells of nonocular surface origin. Epidermal progenitor cells could be provided as a new substitute for conjunctival epithelial cells to overcome the problems of autologous conjunctiva shortage.
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影响因子：
6.5
作者：
Akiyama, M;Smith, LT;Shimizu, H
通讯作者：
Shimizu, H
影响因子：
2.8
作者：
Fukuda, K;Chikama, T;Nishida, T
通讯作者：
Nishida, T
影响因子：
1.5
作者：
Burman, Sanghamitra;Tejwani, Sushma;Sangwan, Virender S.
通讯作者：
Sangwan, Virender S.
影响因子：
4.4
作者：
Ang, LPK;Tan, DTH;Lavker, RM
通讯作者：
Lavker, RM
DOI：
10.1007/s11427-007-0006-4
发表时间：
2007-02-01
期刊：
SCIENCE IN CHINA SERIES C-LIFE SCIENCES
影响因子：
--
作者：
Gao Nan;Wang ZhiChong;Cui GuangHui
通讯作者：
Cui GuangHui