Growth of hodgkin cell lines in severely combined immunodeficient mice
Growth of hodgkin cell lines in severely combined immunodeficient mice
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霍奇金细胞系在严重联合免疫缺陷小鼠中的生长
DOI：
10.1002/ijc.2910520610
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发表时间：
1992
影响因子：
6.4
通讯作者：
V. Diehl
中科院分区：
文献类型：
--
作者：
C. von Kalle;J. Wolf;Andreas Becker;Andrea Sckaer;M. Munck;A. Engert;U. Kapp;C. Fonatsch;D. Komitowski;W. F. de Lacroix;V. Diehl
没有霍奇金淋巴瘤衍生细胞体内生长的动物模型。未经处理的霍奇金病（HD）衍生细胞系和原代活检组织均不能在裸鼠中生长。由于严重联合免疫缺陷（SCID）小鼠已被报道是一个更好的受体移植的人淋巴组织比裸鼠，我们测试了SCID小鼠是否提供了合适的条件，在体内生长的HD细胞系。在未处理和预处理的SCID小鼠以及另一种联合免疫缺陷小鼠品系米色/裸/X-连锁免疫缺陷（BNX）小鼠中检测了HD细胞的致瘤性。SCID小鼠支持测试的6种HD细胞系（L428、L540、L591、DEV、HD-LM 2、KM-H2）的体内生长。在BNX小鼠中，6个细胞系中只有一个细胞系（DEV）具有致瘤性。HD细胞系在T细胞缺陷裸鼠中无增殖。因此，HD细胞系的体内生长似乎与宿主免疫缺陷的程度有关。其他生长支持治疗，如纤维肉瘤共移植、腹膜内矿物油注射或免疫抑制预处理（抗去唾液酸-GMI-抗体注射），允许BNX小鼠中另外3种HD细胞系生长。移植细胞的免疫表型和核型与原细胞系一致。我们的实验描述了一种用于霍奇金病衍生细胞系生长的有效且可重现的异种移植模型。这可能对阐明霍奇金淋巴瘤衍生细胞的生长特征以及测试新的治疗方案具有价值。© 1992 Wiley利斯公司
No animal model exists for the in vivo growth of Hodgkin's‐lymphoma‐derived cells. Neither unmanipulated Hodgkin's‐disease(HD)‐derived cell lines nor primary biopsy tissue could be grown in nude mice. Since the severe combined immunodeficient (SCID) mouse has been reported to be a better recipient for transplanted human lymphatic tissue than the nude mouse, we tested whether SCID mice provide suitable conditions for the in vivo growth of HD cell lines. Tumorigenicity of HD cells was tested in untreated and pre‐treated SCID mice and in another combined immunodeficient mouse strain, beige/nude/X‐linked immunodeficient (BNX) mouse. SCID mice supported in vivo growth of the 6 HD cell lines tested (L428, L540, L591, DEV, HD‐LM2, KM‐H2). Only one of the 6 lines (DEV) was tumorigenic in BNX mice. No HD cell line proliferated in T‐cell‐deficient nude mice. Thus, in vivo growth of HD cell lines appeared to be related to the degree of host immunodeficiency. Additional growth supportive treatments such as fibrosarcoma co‐transplantation, intraperitoneal mineral oil injection or immunosuppressive pre‐treatment (anti‐asialo‐GMI ‐antibody injection) permitted growth of 3 additional HD cell lines in BNX mice. The immunophenotype and karyotype of explanted graft cells were identical to the original cell lines. Our experiments describe an effective and reproducible xenograft model for growth of Hodgkin's‐disease‐derived cell lines. This may be of value for elucidating the growth characteristics of Hodgkin's‐lymphoma‐derived cells as well as for testing new therapeutic regimens. © 1992 Wiley‐Liss, Inc.
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影响因子：
56.9
作者：
MCCUNE, JM;NAMIKAWA, R;WEISSMAN, IL
通讯作者：
WEISSMAN, IL
影响因子：
20.3
作者：
E. Waller;O. Kamel;M. Cleary;A. Majumdar;M. Schick;M. Lieberman;I. Weissman
通讯作者：
E. Waller;O. Kamel;M. Cleary;A. Majumdar;M. Schick;M. Lieberman;I. Weissman
与高剂量单独和联合使用塞替派相关的临床前研究。
影响因子：
4
作者：
Teicher,BA;Holden,SA;Eder,JP;Herman,TS;Antman,KH;Frei3rd,E
通讯作者：
Frei3rd,E
人类白血病细胞系成功移植到裸鼠体内：优化移植物接受的条件。
DOI：
--
发表时间：
1982
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
Ziegler,HW;Frizzera,G;Bach,FH
通讯作者：
Bach,FH