Selective expression of electrical correlates of differential appetitive classical conditioning in a feeding network.
Selective expression of electrical correlates of differential appetitive classical conditioning in a feeding network.
复制标题
喂养网络中差异食欲经典调节的电相关性的选择性表达。
DOI：
--
复制
发表时间：
2001
影响因子：
2.5
通讯作者：
P. Benjamin
中科院分区：
文献类型：
--
作者：
N. Jones;G. Kemenes;P. Benjamin
电相关的差分食欲经典条件反射记录在神经网络的基础上，在蜗牛滞流喂养。在间隔训练（15个试验超过3天），嘴唇和肌腱被用作CS+（强化条件刺激）或CS-（非强化条件刺激）网站的行为触觉条件。在一组实验动物中，触摸嘴唇（CS+部位）之后是蔗糖（非条件刺激，US），但触摸腱（CS-部位）没有得到加强。在第二个实验组中，CS+/CS-位点被颠倒。从实验组和未处理对照组制备半完整的唇-肌腱-CNS制剂。细胞内记录从B3运动神经元的喂养网络，这使得监测活动的喂养中央模式发生器（CPG）的中间神经元，以及早期突触输入诱发的触摸刺激。成功的行为条件反射后，触摸刺激诱发CPG驱动的虚构的喂养活动在CS+，但不是CS-网站在两个实验组。未处理的蜗牛/制剂未显示触摸反应。一个弱的非对称刺激概括条件喂养没有保留在电生理水平。一个早期兴奋性突触后电位（EPSP）响应触摸后，只增强了条件反射的唇CS+/触手CS-组，但不是在触手CS+/唇CS-组。结果表明，差异性食欲经典条件反射的主要特征可以在电生理水平上被记录下来，但条件反射的某些特征在还原制剂中被选择性地表达。
Electrical correlates of differential appetitive classical conditioning were recorded in the neural network that underlies feeding in the snail Lymnaea stagnalis. In spaced training (15 trials over 3 days), the lips and the tentacle were used as CS+ (reinforced conditioned stimulus) or CS- (nonreinforced conditioned stimulus) sites for behavioral tactile conditioning. In one group of experimental animals, touch to the lips (the CS+ site) was followed by sucrose (the unconditioned stimulus, US), but touch to the tentacle (the CS- site) was not reinforced. In a second experimental group the CS+/CS- sites were reversed. Semi-intact lip-tentacle-CNS preparations were made from both experimental groups and a naive control group. Intracellular recordings were made from the B3 motor neuron of the feeding network, which allowed the monitoring of activity in the feeding central pattern generator (CPG) interneurons as well as early synaptic inputs evoked by the touch stimulus. Following successful behavioral conditioning, the touch stimulus evoked CPG-driven fictive feeding activity at the CS+ but not the CS- sites in both experimental groups. Naive snails/preparations showed no touch responses. A weak asymmetrical stimulus generalization of conditioned feeding was not retained at the electrophysiological level. An early excitatory postsynaptic potential (EPSP) response to touch was only enhanced following conditioning in the Lip CS+/tentacle CS- group but not in the Tentacle CS+/lip CS- group. The results show that the main features of differential appetitive classical conditioning can be recorded at the electrophysiological level, but some characteristics of the conditioned response are selectively expressed in the reduced preparation.
单个感觉神经元的联想调节暗示了学习的细胞机制。
DOI：
10.1126/science.6294834
发表时间：
1983
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Walters,ET;Byrne,JH
通讯作者：
Byrne,JH
在简化的外套膜器官制备中，海兔退鳃反射的经典条件反射、差异条件反射和二阶条件反射。
影响因子：
1.9
作者：
Hawkins,RD;Greene,W;Kandel,ER
通讯作者：
Kandel,ER