Distribution of keratin and laminin in ameloblastoma. Comparison with developing tooth and epidermoid carcinoma.
Distribution of keratin and laminin in ameloblastoma. Comparison with developing tooth and epidermoid carcinoma.
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角蛋白和层粘连蛋白在成釉细胞瘤中的分布。
DOI：
10.1111/j.1600-0714.1984.tb01404.x
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
P. Ekblom
中科院分区：
文献类型：
--
作者：
I. Thesleff;P. Ekblom
应用免疫组织化学方法对5例成釉细胞瘤的肿瘤细胞性质进行了研究，并与发育中的小鼠、人牙齿及5例口腔肿瘤进行了比较。所研究的抗原是角蛋白，一种典型的上皮细胞内细胞骨架蛋白，和层粘连蛋白，一种发现于基底膜的细胞外基质蛋白。我们的研究结果表明，角蛋白在成釉细胞瘤、表皮样癌和发育中的牙齿中所有类型的上皮细胞中都有表达。成釉细胞瘤的上皮细胞，角蛋白阳性肿瘤岛被层粘连蛋白连续线包围，其模式与发育中的牙齿相似。在表皮样癌周围也可见层粘连蛋白，但在间质和肿瘤上皮之间经常可见大面积层粘连蛋白缺失。这表明恶性表皮样癌缺乏适当的基底膜形成。这可能是由于癌细胞基底膜蛋白的产生减少或降解增加所致。我们的结果与成釉细胞瘤来源于牙源性上皮细胞的观点一致。角蛋白免疫染色不能区分癌和成釉细胞瘤。然而，基底膜蛋白如层粘连蛋白的可视化可以明显地用于成釉细胞瘤和癌的鉴别诊断。
The nature of the tumor cells in 5 cases of ameloblastomas was studied by immunohistochemistry, and the findings were compared with developing mouse and human teeth as well as with 5 cases of carcinomas in the oral region. The antigens investigated were keratin, an intracellular cytoskeletal protein typical of epithelial cells, and laminin, an extracellular matrix protein found in basement membranes. Our results show that keratin is expressed by all types of epithelial cells in ameloblastomas as well as in the epidermoid carcinomas, and developing teeth. The epithelial, keratin-positive tumor islands in the ameloblastomas were surrounded by a continuous line of laminin, in a pattern similar to that seen in developing tooth. Laminin was seen also around the epidermoid carcinomas but large areas devoid of laminin were constantly seen between the stroma and the neoplastic epithelium. This indicates a lack of proper basement membrane formation by the malignant epidermoid carcinomas. This may be due either to a diminished production or an increased degradation of basement membrane proteins by the carcinoma cells. Our results are in line with suggestions that ameloblastomas are derived from odontogenic epithelial cells. Immunostaining for keratin does not distinguish between carcinomas and the ameloblastomas. However, visualization of basement membrane proteins such as laminin can apparently be used in the differential diagnosis between ameloblastomas and carcinomas.
DOI：
10.1073/pnas.78.6.3901
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
INGBER, DE;MADRI, JA;JAMIESON, JD
通讯作者：
JAMIESON, JD